Endonuclease cleavage combined with horseradish peroxidase-assisted signal amplification for electrochemical monitoring of DNA
| Autor: | Tianxiang Wei, Wenwen Tu, Min Han, Can Zhang, Zhihui Dai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Detection limit
biology Chemistry Cleavage (embryo) biology.organism_classification Horseradish peroxidase Mycobacterium tuberculosis lcsh:Chemistry Endonuclease chemistry.chemical_compound Biochemistry lcsh:Industrial electrochemistry lcsh:QD1-999 Electrochemistry biology.protein DNA Peroxidase Conjugate lcsh:TP250-261 |
| Zdroj: | Electrochemistry Communications, Vol 22, Iss, Pp 133-136 (2012) |
| ISSN: | 1388-2481 |
| Popis: | A novel and universal electrochemical strategy for monitoring DNA was developed based on specific cleavage of MspI endonuclease combining with streptavidin–horseradish peroxidase (SA–HRP) conjugate. By introducing the strategy to mycobacterium tuberculosis (MTB) DNA detection, the probe DNA was immobilized on Au electrode via AuS bond and SA–HRP conjugate was linked to probe DNA by biotin–SA specific interaction, which led to the first application in MTB DNA electrochemical detection. This proposed electrochemical method exhibited satisfactory performance which could detect MTB DNA linearly ranging from 10 pM to 100 nM with a detection limit of 2.3 pM. The novel strategy of DNA analysis showed a promising application in clinic diagnostics. Keywords: Electrochemistry, DNA, Endonuclease, Streptavidin–horseradish peroxidase, Mycobacterium tuberculosis |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect