Magnetic resonance imaging provides sensitive in vivo assessment of experimental ventilator-induced lung injury
| Autor: | Laura E. Fredenburgh, Raúl San José Estépar, Dean O. Kuethe, Rebecca M. Baron, George R. Washko, Jeremy M. Hix, Piotr T. Filipczak, Andrew P. Gigliotti |
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| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Male Pulmonary and Respiratory Medicine ARDS Pathology medicine.medical_specialty Physiology Ventilator-Induced Lung Injury medicine.medical_treatment Respiratory physiology Lung injury 030218 nuclear medicine & medical imaging Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Airway resistance In vivo Physiology (medical) Animals Medicine Lung Mechanical ventilation medicine.diagnostic_test business.industry Airway Resistance Magnetic resonance imaging Cell Biology respiratory system medicine.disease Magnetic Resonance Imaging Respiration Artificial respiratory tract diseases medicine.anatomical_structure Neutrophil Infiltration Call for Papers business 030217 neurology & neurosurgery |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 311:L208-L218 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00459.2015 |
| Popis: | Animal models play a critical role in the study of acute respiratory distress syndrome (ARDS) and ventilator-induced lung injury (VILI). One limitation has been the lack of a suitable method for serial assessment of acute lung injury (ALI) in vivo. In this study, we demonstrate the sensitivity of magnetic resonance imaging (MRI) to assess ALI in real time in rat models of VILI. Sprague-Dawley rats were untreated or treated with intratracheal lipopolysaccharide or PBS. After 48 h, animals were mechanically ventilated for up to 15 h to induce VILI. Free induction decay (FID)-projection images were made hourly. Image data were collected continuously for 30 min and divided into 13 phases of the ventilatory cycle to make cinematic images. Interleaved measurements of respiratory mechanics were performed using a flexiVent ventilator. The degree of lung infiltration was quantified in serial images throughout the progression or resolution of VILI. MRI detected VILI significantly earlier (3.8 ± 1.6 h) than it was detected by altered lung mechanics (9.5 ± 3.9 h, P = 0.0156). Animals with VILI had a significant increase in the Index of Infiltration ( P = 0.0027), and early regional lung infiltrates detected by MRI correlated with edema and inflammatory lung injury on histopathology. We were also able to visualize and quantify regression of VILI in real time upon institution of protective mechanical ventilation. Magnetic resonance lung imaging can be utilized to investigate mechanisms underlying the development and propagation of ALI, and to test the therapeutic effects of new treatments and ventilator strategies on the resolution of ALI. |
| Databáze: | OpenAIRE |
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