Naoxintong accelerates diabetic wound healing by attenuating inflammatory response
Autor: | Hong Shi, Qianyi Wang, Shaoxia Wang, Leyu Fang, Wei Sun, Lu Chen, Juan He, Hong Wang, Lusha Zhang, Min Song, Wenjie Yang, Yuze Leng, Chunxiao Li, Xiumei Gao |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Male
STAT3 Transcription Factor ecm remoulding Inflammatory response macrophage polarization Macrophage polarization Pharmaceutical Science Traditional Chinese medicine Pharmacology 030226 pharmacology & pharmacy 01 natural sciences Diabetes Mellitus Experimental 03 medical and health sciences Mice 0302 clinical medicine Drug Discovery Medicine Animals Efferocytosis Inflammation efferocytosis Wound Healing integumentary system business.industry Macrophages lcsh:RM1-950 General Medicine 0104 chemical sciences Extracellular Matrix 010404 medicinal & biomolecular chemistry lcsh:Therapeutics. Pharmacology Complementary and alternative medicine Diabetic wound healing Molecular Medicine business Wound healing STAT6 Transcription Factor Drugs Chinese Herbal Research Article |
Zdroj: | Pharmaceutical Biology, Vol 59, Iss 1, Pp 252-261 (2021) Pharmaceutical Biology article-version (VoR) Version of Record |
ISSN: | 1744-5116 1388-0209 |
Popis: | Context Naoxintong (NXT), a prescribed traditional Chinese medicine, widely used in cerebrovascular and cardiovascular diseases, could be effective in diabetic wounds. Objective This study evaluates the wound healing activity of NXT by employing an excisional wound splinting model. Materials and methods NXT was dissolved in saline and given daily by gavage. Wounds were induced at the dorsum of non-diabetic (db/+) and diabetic (db/db) mice and treated with saline or 700 mg/kg/d NXT for 16 days. Wound closure was measured every four days. Extracellular matrix (ECM) remodelling, collagen deposition, leukocyte infiltration and expression of Col-3, CK14, CXCL1, CXCL2, MPO, Ly6G, CD68, CCR7, CD206, p-JAK1, p-STAT3 and p-STAT6 was analysed. Results NXT significantly accelerated rate of wound closure increased from 70% to 84%, accompanied by up-regulation of collagen deposition and ECM at days 16 post-injury. Moreover, NXT alleviated neutrophil infiltration, accompanied by down-regulation of CXCL1 and CXCL2 mRNA expression. In addition, NXT markedly augmented neutrophil efferocytosis. In diabetic wounds, the levels of M1 marker gene (CCR7) increased, while M2 marker gene (CD206) decreased, demonstrating a pro-inflammatory shift. Application of NXT increased M2 macrophage phenotype in db/db mice. Mechanistically, NXT treatment increased expression level of p-STAT3 and p-STAT6 at days 3 post-injury, indicating NXT mediated macrophages towards M2 phenotype and alleviated inflammation in diabetic wounds by activation of STAT3 and STAT6. Conclusions Our study provides evidence that NXT accelerates diabetic wound healing by attenuating inflammatory response, which provides an important basis for use of NXT in the treatment of chronic diabetic wound healing. |
Databáze: | OpenAIRE |
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