Evaluation of 5 serologic assays to detect Rhodococcus equi pneumonia in foals
| Autor: | Noah D. Cohen, Shinji Takai, Arthur B. Angulo, Charity L. Doherty, Ronnie F. Edwards, Ronald J. Martens, M. Keith Chaffin |
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| Rok vydání: | 2002 |
| Předmět: |
Immunodiffusion
animal diseases Enzyme-Linked Immunosorbent Assay Hemolytic Plaque Technique Hemolysis Inhibition Sensitivity and Specificity digestive system Serology Paired samples Rhodococcus equi parasitic diseases Pneumonia Bacterial medicine Animals Horses General Veterinary biology business.industry biology.organism_classification Ouchterlony double immunodiffusion medicine.disease Antibodies Bacterial Pneumonia Case-Control Studies Immunology Horse Diseases business Actinomycetales Infections |
| Zdroj: | Journal of the American Veterinary Medical Association. 221:825-833 |
| ISSN: | 0003-1488 |
| DOI: | 10.2460/javma.2002.221.825 |
| Popis: | Objective—To determine the sensitivity and specificity of 5 serologic assays used to diagnose Rhodococcus equi pneumonia in foals and to determine whether any of the assays could be used to identify affected foals prior to the onset of clinical signs or to differentiate between affected and unaffected foals when clinical signs first become apparent. Design—Nested case-control study. Animals—26 foals. Procedure—Serum samples were obtained from all foals at 2, 4, and 6 or 7 weeks of age. Additional samples were obtained from affected foals at the time of diagnosis of R equi pneumonia and from agematched unaffected foals. Samples were tested with 3 ELISA, an agar gel immunodiffusion assay, and a synergistic hemolysis inhibition assay. Results—Sensitivity and specificity data indicated that none of the assays could be used to reliably differentiate affected from unaffected foals at any testing period. Proportions of foals that had an increase in test values between paired samples collected at 4 and 6 or 7 weeks of age were not significantly different between affected and unaffected foals. For all assays, result values increased significantly over time; however, the rate of increase was not significantly different between affected and unaffected foals. Conclusions and Clinical Relevance—Results suggest that serologic assays, whether performed on single or paired samples, cannot be used to reliably establish, confirm, or exclude a diagnosis of R equi pneumonia in foals. (J Am Vet Med Assoc 2002;221:825–833) |
| Databáze: | OpenAIRE |
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