Monitoring the Activity and Inhibition of Cholinesterase Enzymes using Single-Walled Carbon Nanotube Fluorescent Sensors

Autor:	Dan Loewenthal, Dotan Kamber, Gili Bisker
Rok vydání:	2022
Předmět:	Nanotubes Carbon Butyrylcholinesterase Acetylcholinesterase Cholinesterase Inhibitors Pesticides Nerve Agents Biomarkers Analytical Chemistry
Zdroj:	Analytical Chemistry. 94:14223-14231
ISSN:	1520-6882 0003-2700
Popis:	Cholinesterase enzymes are involved in a wide range of bodily functions, and their disruption is linked to pathologies such as neurodegenerative diseases and cancer. While cholinesterase inhibitors are used as drug treatments for diseases such as Alzheimer and dementia at therapeutic doses, acute exposure to high doses, found in pesticides and nerve agents, can be lethal. Therefore, measuring cholinesterase activity is important for numerous applications ranging from the search for novel treatments for neurodegenerative disorders to the on-site detection of potential health hazards. Here, we present the development of a near-infrared (near-IR) fluorescent single-walled carbon nanotube (SWCNT) optical sensor for cholinesterase activity and demonstrate the detection of both acetylcholinesterase and butyrylcholinesterase, as well as their inhibition. We show sub U L
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ab626b6238682e3c4e57666f6eefc50 https://doi.org/10.1021/acs.analchem.2c02471 Zobrazit plný text záznamu