Culture of the primary corneal epithelium as a potential component of test batteries for eye irritancy testing
| Autor: | Ozga T, Lipski K, Szaflik J, Joanna Olkowska-Truchanowicz, Liberek I, Dariusz Sladowski |
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| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharides
Time Factors genetic structures medicine.medical_treatment Cell Culture Techniques Inflammation CD59 Antigens CD59 Biology Toxicology Animal Testing Alternatives Cornea Toxicity Tests medicine Humans Complement Activation Cells Cultured Corneal epithelium Dose-Response Relationship Drug Epithelium Corneal General Medicine eye diseases Complement system medicine.anatomical_structure Cytokine Immunology Irritants Tears Human eye sense organs medicine.symptom Interleukin-1 |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 19(7) |
| ISSN: | 0887-2333 |
| Popis: | One of the main goals for toxicologists working on the development of in vitro tests is to replace the animal-based eye irritation test. Inflammation is one of the mechanisms which have not been covered sufficiently by the existing in vitro ocular irritancy test systems. As there are major species differences between the human and rabbit eye inflammation mechanisms, the most relevant test system is the human eye itself. The current study focused on an evaluation of the practical availability of human corneal epithelial cells for routine eye irritancy testing. Human corneal epithelium cell cultures were used to assess the effects of lipopolysaccharide on IL-1 beta release. The findings indicated that cytokine release can be augmented by the presence of the complement system, which is normally found in tears. However, the corneal cells were found to be highly resistant to the complement system, which can be attributed to the very high expression of CD59, a powerful complement regulatory protein found in the corneal epithelium. It is estimated that discarded corneas from tissue banks could provide enough material for routine testing by this method. |
| Databáze: | OpenAIRE |
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