The conformational change of rabbit muscle pyruvate kinase induced by activating cations and its substrates
| Autor: | Shaoning Yu, Yun Zhang, Yan Ou, Wenbing Tao, Guangrong Wu |
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| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular Protein Denaturation Conformational change Protein Conformation Pyruvate Kinase Biochemistry Catalysis chemistry.chemical_compound Structural Biology Cations Animals Enzyme Inhibitors Molecular Biology Quenching (fluorescence) biology Chemistry Circular Dichroism Muscles Temperature Tryptophan Active site General Medicine Solvent accessibility Fluorescence Enzyme Activation Spectrometry Fluorescence Acrylamide Solvents biology.protein Biophysics Rabbits Pyruvate kinase |
| Zdroj: | International Journal of Biological Macromolecules. 47:228-232 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2010.04.017 |
| Popis: | Catalysis by rabbit muscle pyruvate kinase involves domain movements and conformational changes induced by activating cations and its substrates. Fluorescence acrylamide quenching analyses reveal that interactions with Mg 2+ and K + lead to a more exposed active site of PK while interactions with PEP and ADP decrease solvent accessibility of the active site. |
| Databáze: | OpenAIRE |
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