Activated gut-homing CD8+ T cells for coeliac disease diagnosis on a gluten-free diet
| Autor: | Natalia López-Palacios, María Corzo, Pedro A. Reche, Mercedes Galindo Rubio, Saúl Horta, Beatriz Arau, Maria Esteve, F Fernández-Bañares, Sergio Farrais, Eva Tristán, Marta Gomez-Perosanz, Juana María Hernández, Marta Fernández-Prieto, Mar Pujals, Concepción Núñez |
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| Rok vydání: | 2021 |
| Předmět: |
Glutens
T cell T cells CD8 T cells CD38 CD8-Positive T-Lymphocytes Coeliac disease IFN-γ ELISPOT Diet Gluten-Free Clinical trials Medicine Cytotoxic T cell Blood test Humans Celiac disease Flow cytometry Gluten challenge medicine.diagnostic_test business.industry ELISPOT General Medicine medicine.disease Celiac Disease medicine.anatomical_structure Dieta sense gluten Cèl·lules T Citometria de fluxe Immunology Gluten-free diet Gluten free Malaltia celíaca business CD8 Research Article Assaigs clínics |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona BMC Medicine BMC Medicine, Vol 19, Iss 1, Pp 1-10 (2021) |
| Popis: | Background The diagnosis of coeliac disease (CD) in individuals that have started a gluten-free diet (GFD) without an adequate previous diagnostic work-out is a challenge. Several immunological assays such as IFN-γ ELISPOT have been developed to avoid the need of prolonged gluten challenge to induce the intestinal damage. We aimed to evaluate the diagnostic accuracy of activated gut-homing CD8+ and TCRγδ+ T cells in blood after a 3-day gluten challenge and to compare it with the performance of IFN-γ ELISPOT in a HLA-DQ2.5 subsample. Methods A total of 22 CD patients and 48 non-CD subjects, all of them following a GFD, underwent a 3-day 10-g gluten challenge. The percentage of two T cell subsets (CD8+ CD103+ β7hi CD38+/total CD8+ and TCRγδ+ CD103+ β7hi CD38+/total TCRγδ+) in fresh peripheral blood drawn baseline and 6 days after the challenge was determined by flow cytometry. IFN-γ ELISPOT assays were also performed in HLA-DQ2.5 participants. ROC curve analysis was used to assess the diagnostic performance of the CD8+ T cell response and IFN-γ ELISPOT. Results Significant differences between the percentage of the two studied subsets of CD8+ and TCRγδ+ cells at days 0 and 6 were found only when considering CD patients (p < 10−3 vs. non-CD subjects). Measuring activated CD8+ T cells provided accurate CD diagnosis with 95% specificity and 97% sensitivity, offering similar results than IFN-γ ELISPOT. Conclusions The results provide a highly accurate blood test for CD diagnosis in patients on a GFD of easy implementation in daily clinical practice. |
| Databáze: | OpenAIRE |
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