A simple and low-cost resazurin assay for vitality assessment across species
| Autor: | Alexander Mehring, Jakob Walther, Niklas Erdmann, Roland Ulber, Dorina Strieth, Judith Stiefelmaier |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sporosarcina Biological organism Biomass Bioengineering Vitality 01 natural sciences Applied Microbiology and Biotechnology Suspension culture 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Oxazines Food science biology Resazurin General Medicine biology.organism_classification Sporosarcina pasteurii Alamar blue 030104 developmental biology chemistry Xanthenes Biological Assay Biotechnology |
| Zdroj: | Journal of biotechnology. 333 |
| ISSN: | 1873-4863 |
| Popis: | Working with biological organisms requires knowledge about the state of their viability and vitality to ascertain efficient processes. The phenoxazine dye resazurin is routinely used for viability assessment of many different species. Here, a novel use for resazurin as an indicator for vitality assessment across several species is proposed. Different amounts of biomass as well as mixtures of live/dead biomass were investigated for their capabilities of metabolizing resazurin and monitored over time. Increasing (live) biomass was found to increase reaction rate in a linear fashion, giving information about the cells' vitality. In an application example, stored suspension cultures of Sporosarcina pasteurii were found to decrease in viability over time, while urease activity decreased as well. For the first time, the assessment of vitality by one technique was demonstrated for several species in parallel. |
| Databáze: | OpenAIRE |
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