Efficient reprogramming of adult neural stem cells to monocytes by ectopic expression of a single gene
| Autor: | Konstantinos Meletis, Maggie S. Y. Yeung, Magda Forsberg, Jonas Frisén, Mats A. A. Persson, Fanie Barnabé-Heider, Marie Carlén, Johan Aarum |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Time Factors Somatic cell Green Fluorescent Proteins Biology Monocytes Cell Line Mice Pregnancy Transduction Genetic Proto-Oncogene Proteins Neurosphere Animals Humans Cells Cultured Neurons Microscopy Confocal Multidisciplinary Stem Cells Calcium-Binding Proteins Lentivirus Microfilament Proteins Biological Sciences Cellular Reprogramming Embryo Mammalian Immunohistochemistry Neural stem cell Cell biology Mice Inbred C57BL Transplantation Trans-Activators Female Ectopic expression Stem cell Reprogramming Adult stem cell |
| Zdroj: | Proceedings of the National Academy of Sciences. 107:14657-14661 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1009412107 |
| Popis: | Neural stem cells have a broad differentiation repertoire during embryonic development and can be reprogrammed to pluripotency comparatively easily. We report that adult neural stem cells can be reprogrammed at very high efficiency to monocytes, a differentiated fate of an unrelated somatic lineage, by ectopic expression of the Ets transcription factor PU.1. The reprogrammed cells display a marker profile and functional characteristics of monocytes and integrate into tissues after transplantation. The failure to reprogram lineage-committed neural cells to monocytes with PU.1 suggests that neural stem cells are uniquely amenable to reprogramming. |
| Databáze: | OpenAIRE |
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