DNA Damage, n-3 Long-Chain PUFA Levels and Proteomic Profile in Brazilian Children and Adolescents
| Autor: | Tamiris Trevisan de Barros, Jacqueline Pontes Monteiro, Maria Olímpia Ribeiro do Vale Almada, Ornella Cominetti, Lusania Maria Greggi Antunes, Lívia Cristina Hernandes, Carolina de Almeida Coelho-Landell, Mariana Giaretta Mathias, Vinicius Paula Venancio, Roseli Borges Donegá Toffano, Fábio da Veiga Ued, José Simon Camelo-Junior, Elaine Hillesheim, Jim Kaput, Sofia Moco, Roberta Garcia Salomão |
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| Přispěvatelé: | Molecular and Computational Toxicology, AIMMS |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Proteomics 0301 basic medicine DNA protection medicine.medical_specialty Adolescent Docosahexaenoic Acids Class I Phosphatidylinositol 3-Kinases Hydrolases DNA damage fatty acids Article ADOLESCENTES 03 medical and health sciences 0302 clinical medicine Cyclin C Internal medicine Fatty Acids Omega-3 Protein Kinase C beta medicine Humans TX341-641 adolescents proteomic Inflammation chemistry.chemical_classification child Nutrition and Dietetics Proteomic Profile Chemistry Nutrition. Foods and food supply Cyclin-Dependent Kinase 8 Eicosapentaenoic acid Class Ia Phosphatidylinositol 3-Kinase Comet assay Cross-Sectional Studies 030104 developmental biology Endocrinology Eicosapentaenoic Acid Docosahexaenoic acid 030220 oncology & carcinogenesis Female Long chain Brazil Food Science Polyunsaturated fatty acid |
| Zdroj: | Nutrients, 13(8):2483, 1-12. Multidisciplinary Digital Publishing Institute (MDPI) Nutrients, Vol 13, Iss 2483, p 2483 (2021) Nutrients Volume 13 Issue 8 Barros, T T, Venâncio, V D P, Hernandes, L C, Greggi Antunes, L M, Hillesheim, E, Salomão, R G, Mathias, M G, Coelho-Landell, C A, Toffano, R B D, Do Vale Almada, M O R, Camelo-Junior, J S, Moco, S, Cominetti, O, Ued, F D V, Kaput, J & Monteiro, J P 2021, ' DNA Damage, n-3 Long-Chain PUFA Levels and Proteomic Profile in Brazilian Children and Adolescents ', Nutrients, vol. 13, no. 8, 2483, pp. 1-12 . https://doi.org/10.3390/nu13082483 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2072-6643 |
| Popis: | Fatty acids play a significant role in maintaining cellular and DNA protection and we previously found an inverse relationship between blood levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and DNA damage. The aim of this study was to explore differences in proteomic profiles, for 117 pro-inflammatory proteins, in two previously defined groups of individuals with different DNA damage and EPA and DHA levels. Healthy children and adolescents (n = 140) aged 9 to 13 years old in an urban area of Brazil were divided by k-means cluster test into two clusters of DNA damage (tail intensity) using the comet assay (cluster 1 = 5.9% ± 1.2 and cluster 2 = 13.8% ± 3.1) in our previous study. The cluster with higher DNA damage and lower levels of DHA (6.2 ± 1.6 mg/dL 5.4 ± 1.3 mg/dL, p = 0.003) and EPA (0.6 ± 0.2 mg/dL 0.5 ± 0.1 mg/dL, p < 0.001) presented increased expression of the proteins CDK8–CCNC, PIK3CA–PIK3R1, KYNU, and PRKCB, which are involved in pro-inflammatory pathways. Our findings support the hypothesis that low levels of n-3 long-chain PUFA may have a less protective role against DNA damage through expression of pro-inflammatory proteins, such as CDK8–CCNC, PIK3CA–PIK3R1, KYNU, and PRKCB. |
| Databáze: | OpenAIRE |
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