In vitro cytogenetic evaluation of the particular combination of flurbiprofen and roxithromycin
| Autor: | Mostafa Norizadeh Tazehkand, Hasan Basri Ila, Mehmet Topaktaş, Mehmet Tahir Husunet, Rima Çelik, Taygun Timocin, Ebrahim Valipour |
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| Přispěvatelé: | Zonguldak Bülent Ecevit Üniversitesi, Çukurova Üniversitesi, Çukurova Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Hüsunet, Mehmet Tahir, Timoçin, Taygun, Tazehkand, Mostafa Norizadeh, Çelik, Rima, Topaktaş, Mehmet, İla, Hasan Basri |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Health Toxicology and Mutagenesis Flurbiprofen Pharmacology Toxicology medicine.disease_cause 03 medical and health sciences Young Adult medicine Cytotoxic T cell Humans micronucleus oxidative stress Drug Interactions Lymphocytes Cytotoxicity Cells Cultured Micronuclei Chromosome-Defective Roxithromycin Chemical Health and Safety Dose-Response Relationship Drug Chemistry Anti-Inflammatory Agents Non-Steroidal genotoxicity Public Health Environmental and Occupational Health General Medicine In vitro Anti-Bacterial Agents 030104 developmental biology Micronucleus test cytotoxicity Female Micronucleus Genotoxicity Cell Division medicine.drug DNA Damage Plasmids |
| Popis: | PubMedID: 27600436 Flurbiprofen (FLB) (anti-inflammatory and analgesic drug) and roxithromycin (RXM) (antibiotic) were widely used in world wide. This study deals with investigation of genotoxicity, cytotoxicity, and oxidative stress effects of a particular combination of these drugs in human cultured lymphocytes. Also, DNA damaging-protective effects of combination of these drugs were analyzed on plasmid DNA. Human lymphocytes were treated with different concentrations (FLB + RXM; 10 µg/mL + 25 µg/mL, 15 µg/mL + 50 µg/mL, and 20 µg/mL + 100 µg/mL) of the drugs following by study of their genotoxic and cytotoxic effects by analysis of cytokinesis-block micronucleus test and nuclear division index, respectively. The effect of the combination in aspect of anti-oxidative and DNA damaging activity was evaluated on Pet-22b plasmid. According to our results, the combination of FLB and RXM did not show a notable genotoxic effect on cells. Although each of the substances had been shown as a cytotoxic agent by previous researchers, in this research, the combination of these drugs did not exhibit any adverse effect on cell division. FLB had DNA protection effect against H2O2 while in combination with RXM had not the same effect on the plasmid. © 2016 Informa UK Limited, trading as Taylor & Francis Group. |
| Databáze: | OpenAIRE |
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