Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation
| Autor: | Giacinto Della Casa, Giancarlo Rossi, Antonella Stoppacciaro, Hongjun Wang, Massimo Sargiacomo, Luigina Renzi, Marialuisa Lavitrano, Giorgio Stassi, Paola Sinibaldi, Monica Forni, Carla Di Stefano, Daniela Fioretti, Eraldo Seren, Valeria Turchi, Gabriella Marfe, Loredana Pucci, Davide Lazzereschi, Maria Laura Bacci, Roberto Giovannoni, Paola Giancotti |
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| Přispěvatelé: | Lavitrano, M, Bacci, M, Forni, M, Lazzereschi, D, Di Stefano, C, Fioretti, D, Giancotti, P, Marfé, G, Pucci, L, Renzi, L, Wang, H, Stoppacciaro, A, Stassi, G, Sargiacomo, M, Sinibaldi, P, Turchi, V, Giovannoni, R, Della Casa, G, Seren, E, Rossi, G |
| Rok vydání: | 2002 |
| Předmět: |
Male
SMGT hyperacute rejection swine hyperacute rejection Transgene Xenotransplantation medicine.medical_treatment Transplantation Heterologous Biology SMGT swine Animals Genetically Modified Sperm-mediated gene transfer Gene expression smgt medicine Animals Humans Settore MED/05 - Patologia Clinica Transgenes Gene Decay-accelerating factor Multidisciplinary CD55 Antigens MED/04 - PATOLOGIA GENERALE Gene Transfer Techniques DNA Biological Sciences Spermatozoa Molecular biology Transplantation Transgenesis |
| Zdroj: | Proceedings of the National Academy of Sciences. 99:14230-14235 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.222550299 |
| Popis: | A large number ofhDAFtransgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying thehDAFgene transcribed it in a stable manner (64%). The great majority of pigs that transcribed the gene expressed the protein (83%). ThehDAFgene was transmitted to progeny. Expression was stable and found incaveolaeas it is in human cells. The expressed gene was functional based onin vitroexperiments performed on peripheral blood mononuclear cells. These results show that our SMGT approach to transgenesis provides an efficient procedure for studies involving large animal models. |
| Databáze: | OpenAIRE |
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