Genome-wide identification, evolutionary relationship and expression analysis of AGO, DCL and RDR family genes in tea
| Autor: | Tapan Kumar Mondal, Pooja Moni Baruah, Debasish B. Krishnatreya, Niraj Agarwala, Bhaskar Dowarah, Soni Chowrasia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Small RNA Science Arabidopsis RNA-dependent RNA polymerase Cell Cycle Proteins Biology Genes Plant 01 natural sciences Article Camellia sinensis Evolution Molecular 03 medical and health sciences Gene Expression Regulation Plant Gene expression Gene family Gene silencing Arabidopsis thaliana Gene Biotic Phylogeny Plant Proteins Genetics Multidisciplinary Abiotic Drought Arabidopsis Proteins food and beverages Argonaute biology.organism_classification RNA-Dependent RNA Polymerase Computational biology and bioinformatics 030104 developmental biology Argonaute Proteins Medicine 010606 plant biology & botany Genome-Wide Association Study |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Three gene families in plants viz. Argonaute (AGOs), Dicer-like (DCLs) and RNA dependent RNA polymerase (RDRs) constitute the core components of small RNA mediated gene silencing machinery. The present study endeavours to identify members of these gene families in tea and to investigate their expression patterns in different tissues and various stress regimes. Using genome-wide analysis, we have identified 18 AGOs, 5 DCLs and 9 RDRs in tea, and analyzed their phylogenetic relationship with orthologs of Arabidopsis thaliana. Gene expression analysis revealed constitutive expression of CsAGO1 in all the studied tissues and stress conditions, whereas CsAGO10c showed most variable expression among all the genes. CsAGO10c gene was found to be upregulated in tissues undergoing high meristematic activity such as buds and roots, as well as in Exobasidium vexans infected samples. CsRDR2 and two paralogs of CsAGO4, which are known to participate in biogenesis of hc-siRNAs, showed similarities in their expression levels in most of the tea plant tissues. This report provides first ever insight into the important gene families involved in biogenesis of small RNAs in tea. The comprehensive knowledge of these small RNA biogenesis purveyors can be utilized for tea crop improvement aimed at stress tolerance and quality enhancement. |
| Databáze: | OpenAIRE |
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