Redefinition of Nosema pyrausta (Perezia pyraustae Paillot 1927) basing upon ultrastructural and molecular phylogenetic studies
| Autor: | Anastasia G. Kononchuk, Irma V. Issi, Andrei N. Frolov, Yuri S. Tokarev, Julia M. Malysh, Elena V. Seliverstova |
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| Rok vydání: | 2014 |
| Předmět: |
European corn borer
China Genes Fungal Zoology Moths Ostrinia Russia Nosema parasitic diseases Botany Parasite hosting Animals DNA Fungal Phylogeny General Veterinary biology Base Sequence fungi Pyrausta Fungal genetics Genes rRNA RNA Fungal General Medicine Ribosomal RNA Spores Fungal biology.organism_classification Microsporidium Europe Infectious Diseases Haplotypes Insect Science Microsporidia North America Parasitology |
| Zdroj: | Parasitology research. 114(2) |
| ISSN: | 1432-1955 |
| Popis: | Populations of European corn borer (Ostrinia nubilalis Hubner) from Krasnodar Territory (Southwestern Russia) become regularly infected with Nosema-like microsporidia. To identify the parasite, it was subjected to electron microscopy and small subunit ribosomal RNA (SSU rRNA) gene sequencing. The spore ultrastructure of the parasite was highly similar to Nosema bombycis from China and Nosema pyrausta from the USA. The nucleotide sequence of SSU rRNA gene was identical to a microsporidium isolated from O. nubilalis in southern France (GenBank accession no. HM566196) and closely related to Nosema bombycis (no. AY209011, 99.7 % sequence similarity) from Bombyx mori of Chinese origin and N. pyrausta (no. AY958071) from O. nubilalis of North American origin. As the molecular haplotype of SSU rRNA is fixed for the parasite infecting O. nubilalis across Europe and N. pyrausta was initially described in France as Perezia pyraustae (Paillot CR Acad Sci Paris 185: 673–675, 1927), we conclude that the parasite examined under the present study correspond to the type isolate of N. pyrausta. The microsporidium from O. nubilalis in North America (no. AY958071) corresponds therefore to a closely related, yet distinct haplotype. |
| Databáze: | OpenAIRE |
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