Genetically expressed voltage sensor ArcLight for imaging large scale cortical activity in the anesthetized and awake mouse
Autor: | Garrett B. Stanley, Peter Y. Borden, Dieter Jaeger, Arthur E. Morrissette, Alex D. Ortiz, Audrey Sederberg, Craig R. Forest, Christian Waiblinger |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Microscope Sensory stimulation therapy Radiological and Ultrasound Technology Errata Neuroscience (miscellaneous) Sensory system Barrel cortex Somatosensory system Cortex (botany) law.invention 03 medical and health sciences 030104 developmental biology 0302 clinical medicine law Radiology Nuclear Medicine and imaging Image sensor Pioneers in Neurophotonics: Special Section Honoring Professor Amiram Grinvald Neuroscience 030217 neurology & neurosurgery Preclinical imaging |
Zdroj: | Neurophotonics. 4(3) |
ISSN: | 2329-423X |
Popis: | With the recent breakthrough in genetically expressed voltage indicators (GEVIs), there has been a tremendous demand to determine the capabilities of these sensors in vivo. Novel voltage sensitive fluorescent proteins allow for direct measurement of neuron membrane potential changes through changes in fluorescence. Here, we utilized ArcLight, a recently developed GEVI, and examined the functional characteristics in the widely used mouse somatosensory whisker pathway. We measured the resulting evoked fluorescence using a wide-field microscope and a CCD camera at 200 Hz, which enabled voltage recordings over the entire cortical region with high temporal resolution. We found that ArcLight produced a fluorescent response in the S1 barrel cortex during sensory stimulation at single whisker resolution. During wide-field cortical imaging, we encountered substantial hemodynamic noise that required additional post hoc processing through noise subtraction techniques. Over a period of 28 days, we found clear and consistent ArcLight fluorescence responses to a simple sensory input. Finally, we demonstrated the use of ArcLight to resolve cortical S1 sensory responses in the awake mouse. Taken together, our results demonstrate the feasibility of ArcLight as a measurement tool for mesoscopic, chronic imaging. |
Databáze: | OpenAIRE |
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