Perivascular Adipose Tissue Inflammation and Coronary Artery Disease: An Autopsy Study Protocol
| Autor: | Luiz Fernando Ferraz da Silva, Renata Eloah de Lucena Ferretti-Rebustini, Aline Nishizawa, Renata Elaine Paraizo Leite, Lea T. Grinberg, Fernanda Marinho Campos, Daniela Souza Farias-Itao, Claudia K. Suemoto, Wilson Jacob Filho, Carlos Augusto Pasqualucci, Karen Cristina Souza da Silva |
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| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty T lymphocytes Adipose tissue Autopsy Inflammation 030204 cardiovascular system & hematology Proinflammatory cytokine Coronary artery disease 03 medical and health sciences 0302 clinical medicine Protocol Medicine business.industry General Medicine medicine.disease Pathophysiology adipose tissue macrophages Coronary arteries medicine.anatomical_structure Atheroma inflammation medicine.symptom atherosclerosis business 030217 neurology & neurosurgery coronary artery disease B lymphocytes |
| Zdroj: | JMIR Research Protocols |
| ISSN: | 1929-0748 |
| Popis: | Background: Perivascular adipose tissue (PAT) inflammation may have a role in coronary artery disease (CAD) pathophysiology. However, most evidence has come from samples obtained during surgical procedures that may imply in some limitations. Moreover, the role of B lymphocytes and inflammation in PAT that is adjacent to unstable atheroma plaques has not been investigated in humans using morphometric measurements. Objective: The objective of this study is to investigate the inflammation in PAT, subcutaneous, and perirenal adipose tissues (SAT and PrAT) among chronic CAD, acute CAD, and control groups in an autopsy study. Methods: Heart, SAT, and PrAT samples are collected from autopsied subjects in a general autopsy service, with the written informed consent of the next-of-kin (NOK). Sociodemographic and clinical data are obtained from a semistructure interview with the NOK. Coronary arteries are dissected and PAT are removed. Sections with the greatest arterial obstruction or unstable plaques, and the local with absence of atherosclerosis in all coronary arteries are sampled. PAT are represented adjacent to these fragments. Adipose tissues are fixed in 4% buffered paraformaldehyde solution and analyzed immunohistochemically for macrophages (CD68), macrophage polarization (CD11c for proinflammatory and CD206 for anti-inflammatory), B lymphocytes (CD20), and T lymphocytes (CD3). Slides will be scanned, and inflammatory cells will be quantified in 20 random fields. Participants will be categorized in CAD groups, after morphometric measurement of arterial obstruction and plaque composition analysis in accordance with American Heart Association classification. Three study groups will be investigated: acute CAD (at least one unstable plaque); chronic CAD (≥50% arterial obstruction); and controls ( |
| Databáze: | OpenAIRE |
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