Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates

Autor: Rohana P. Dassanayake, Shollie M. Falkenberg, Eduardo Casas, Gian Mario De Mia, Richard Booth, Hao Ma, Ana Cristina S. Mosena, John D. Neill, Cláudio Wageck Canal, Matthias Schweizer
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Antigenic characterization
animal diseases
viruses
Principal component analysis
610 Medicine & health
complex mixtures
Virus
Antigen
Phylogenetics
Virology
Animals
Diarrhea Virus 2
Bovine Viral
Análise multivariada
Phylogeny
Genetics
Vaccines
Genetic diversity
Diarrhea Viruses
Bovine Viral
biology
630 Agriculture
Vírus da diarréia viral bovina tipo 2
Diarrhea Virus 1
Bovine Viral
Vírus da diarréia viral bovina tipo 1
Pestivirus
Dendrogram
virus diseases
Testes de neutralização
Análise de componente principal
biochemical phenomena
metabolism
and nutrition
500 Science
biology.organism_classification
Virus neutralization
Antígenos virais
Vaccination
Cross neutralization
Titer
Vacinas
Multivariate Analysis
570 Life sciences
590 Animals (Zoology)
Bovine Virus Diarrhea-Mucosal Disease
Cattle
Bovine viral diarrhea virus
Zdroj: Mosena, Ana Cristina S; Falkenberg, Shollie M; Ma, Hao; Casas, Eduardo; Dassanayake, Rohana P; Booth, Richard; De Mia, Gian Mario; Schweizer, Matthias; Canal, Cláudio W; Neill, John D (2022). Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates. Journal of virological methods, 299, p. 114328. Elsevier 10.1016/j.jviromet.2021.114328
Repositório Institucional da UFRGS
Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
Popis: Bovine viral diarrhea virus (BVDV) comprises two species, BVDV-1 and BVDV-2. But given the genetic diversity among pestiviruses, at least 22 subgenotypes are described for BVDV-1 and 3-4 for BVDV-2. Genetic characterization is generally accomplished through complete or partial sequencing and phylogeny, but it is not a reliable method to define antigenic relationships. The traditional method for evaluating antigenic relationships between pestivirus isolates is the virus neutralization (VN) assay, but interpretation of the data to define antigenic relatedness can be difficult to discern for BVDV isolates within the same BVDV species. Data from this study utilized a multivariate analysis for visualization of VN results to analyze the antigenic relationships between US vaccine strains and field isolates from Switzerland, Italy, Brazil, and the UK. Polyclonal sera were generated against six BVDV strains currently contained in vaccine formulations, and each serum was used in VNs to measure the titers against seven vaccine strains (including the six homologous strains) and 23 BVDV field isolates. Principal component analysis (PCA) was performed using VN titers, and results were interpreted from PCA clustering within the PCA dendrogram and scatter plot. The results demonstrated clustering patterns among various isolates suggesting antigenic relatedness. As expected, the BVDV-1 and BVDV-2 isolates did not cluster together and had the greatest spatial distribution. Notably, a number of clusters representing antigenically related BVDV-1 subgroups contain isolates of different subgenotypes. The multivariate analysis may be a method to better characterize antigenic relationships among BVDV isolates that belong to the same BVDV species and do not have distinct antigenic differences. This might be an invaluable tool to ameliorate the composition of current vaccines, which might well be important for the success of any BVDV control program that includes vaccination in its scheme.
Databáze: OpenAIRE
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