Protective factors in the detoxication of hydrogen peroxide in erythrocytes
| Autor: | N. V. Paniker, G. Y. N. Iyer |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Azides
Erythrocytes Glutathione reductase Guinea Pigs chemistry.chemical_compound Hemoglobins Dogs Animals Humans Cysteine Sulfhydryl Compounds Hexosephosphates Hydrogen peroxide Methemoglobin Carbon Isotopes biology Goats General Medicine Glutathione Haplorhini Hydrogen Peroxide Triazoles Catalase Glucose chemistry Biochemistry Ethylmaleimide Iodoacetamide biology.protein Cats Lactates Sodium azide Cattle Hemoglobin Azide Rabbits |
| Zdroj: | Canadian journal of biochemistry. 47(4) |
| ISSN: | 0008-4018 |
| Popis: | The scope of catalase and of glutathione as factors protecting the hemoglobin of erythrocytes of several animal species against oxidation by hydrogen peroxide has been studied. In catalase-rich cells, catalase by itself can largely protect the hemoglobin even if glutathione is immobilized with iodoacetamide. The protective effect of added glucose was studied in erythrocytes exposed to hydrogen peroxide in the presence of the catalase inhibitors 3-amino-1,2,4-triazole and sodium azide. Except in the cow erythrocytes, glucose is uniformly effective in protecting hemoglobin and also in maintaining glutathione in the presence of aminotriazole. Lactate is generally ineffective in protecting hemoglobin or maintaining glutathione. Inhibition of catalase by azide makes the hemoglobin of monkey erythrocytes quite vulnerable to oxidation by hydrogen peroxide even in the presence of added glucose, but rabbit hemoglobin is effectively protected under the same conditions. However, glutathione levels are maintained in both. Iodoacetamide causes increased methemoglobin formation in glutathione-free hemolysates exposed to hydrogen peroxide in the presence of azide. The effect is largest in the rabbit and guinea pig hemolysates, while it is least in the monkey hemolysate. Cysteine and glutathione can reverse this effect. It would appear that besides catalase and glutathione, a nonglutathione sulfhydryl compound(s) might have a role in the protection of hemoglobin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|