The diversity of intestinal microbiota of Mongolians living in Inner Mongolia, China
| Autor: | Zhuang Guo, Z Sun, Q Huo, D Huo, Jiachao Zhang, Jianmin Qiao, W Huang, Yi Zheng, Qimu Gesudu, Lai-Yu Kwok, Heping Zhang |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Lactobacillus casei China Bifidobacterium longum Firmicutes Zoology Gut flora Real-Time Polymerase Chain Reaction digestive system Microbiology fluids and secretions Lactobacillus otorhinolaryngologic diseases Humans Bifidobacterium biology Bacteria Denaturing Gradient Gel Electrophoresis food and beverages Bacteroidetes Feeding Behavior Sequence Analysis DNA biology.organism_classification Biota Bifidobacterium animalis Gastrointestinal Tract sense organs |
| Zdroj: | Beneficial microbes. 4(4) |
| ISSN: | 1876-2891 |
| Popis: | The Mongolian nationality has developed their unique lifestyle and dietary habit for thousands of years. However, by now, little research has been focused on Mongolian gut microbiota and how it is related to different dietary habits. In this study, denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction (qPCR) methods were applied to reveal the diversity of predominant gut bacteria of 48 healthy Mongolians recruited from Hohhot city and the Xilin Gol pasturing area in Inner Mongolia. Compared to similar studies of other nationalities, results from the present study have confirmed that the composition of Mongolian gut microbiota is highly similar at the phylum level (Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria) but variable at the genus level. Especially, the numbers of Phascolarctobacterium, Lactobacillus and Bifidobacterium are rather high. DGGE profiles of Lactobacillus and Bifidobacterium revealed that Lactobacillus casei, Bifidobacterium longum and Bifidobacterium animalis subsp. lactis were predominant in the gut of the Mongolian subjects studied. On the contrary, Lactobacillus helveticus was detected in every pasturing area Mongolian, but not in any of the Hohhot city Mongolians. qPCR results revealed that the numbers of Lactobacillus and Bifidobacterium of Xilin Gol Mongolians were significantly higher (P |
| Databáze: | OpenAIRE |
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