ADAR Regulates RNA Editing, Transcript Stability, and Gene Expression
| Autor: | Ming Wu, Vivian G. Cheung, Elizabeth So, Yue Zhao, Isabel X. Wang, James L. Devlin |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Genetics
Regulation of gene expression B-Lymphocytes Adenosine Deaminase Intron RNA-dependent RNA polymerase RNA RNA-Binding Proteins Biology Transfection General Biochemistry Genetics and Molecular Biology Article ELAV-Like Protein 1 RNA silencing ELAV Proteins Gene Expression Regulation lcsh:Biology (General) RNA editing Gene expression ADAR Humans RNA Editing RNA Messenger lcsh:QH301-705.5 |
| Zdroj: | Cell Reports, Vol 5, Iss 3, Pp 849-860 (2013) Cell reports |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2013.10.002 |
| Popis: | SUMMARY Adenosine deaminases acting on RNA (ADARs) convert adenosine to inosine, which is then recognized as guanosine. To study the role of ADAR proteins in RNA editing and gene regulation, we sequenced and compared the DNA and RNA of human B cells. Then, we followed up the findings experimentally with siRNA knockdown and RNA and protein immunoprecipitations. The results uncovered over 60,000 A-to-G editing sites and several thousand genes whose expression levels are influenced by ADARs. Of these ADAR targets, 90% were identified. Our results also reveal that ADAR regulates transcript stability and gene expression through interaction with HuR (ELAVL1). These findings extend the role of ADAR and show that it cooperates with other RNA-processing proteins to regulate the sequence and expression of transcripts in human cells. |
| Databáze: | OpenAIRE |
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