Detection of respiratory pathogens in porcine lung tissue and lavage fluid
| Autor: | Elisabeth grosse Beilage, Joachim Spergser, Regina Tegeler, Lars Moorkamp, Heiko Nathues |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Swine animal diseases Streptococcus suis Microbiology Mycoplasma hyopneumoniae otorhinolaryngologic diseases medicine Animals Respiratory system Pasteurella multocida Actinobacillus pleuropneumoniae Lung Swine Diseases Bordetella bronchiseptica General Veterinary medicine.diagnostic_test biology Bacteria respiratory system biology.organism_classification respiratory tract diseases Bronchoalveolar lavage medicine.anatomical_structure Animal Science and Zoology Bronchoalveolar Lavage Fluid |
| Zdroj: | Veterinary journal (London, England : 1997). 175(2) |
| ISSN: | 1090-0233 |
| Popis: | The objective of this study was to compare the detection rate of bacterial agents in bronchoalveolar lavage fluid (BALF), taken without visual control, to that in affected lung tissue obtained from the same pig at necropsy. BALF and affected lung tissue were examined for Mycoplasma hyopneumoniae using PCR, and standard cultural methods were used for Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Haemophilus parasuis, Pasteurella multocida and Streptococcus suis. All pigs with a history of respiratory symptoms were submitted as live animals for routine diagnostic examination. In each animal the site of lavage, marked by injecting methylene blue, differed from the site of pneumonic lesions. M. hyopneumoniae was detected more frequently in lung tissue than in BALF in cases with moderate or severe lung lesions. The detection rates of M. hyopneumoniae were higher in the BALF of pigs with mild lesions. Cultural examination of BALF was at least as satisfactory as affected lung tissue for detecting B. bronchiseptica, H. parasuis and P. multocida. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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