Expression of importin-α isoforms in human nasal mucosa: implication for adaptation of avian influenza A viruses to human host

Autor: Prasert Auewarakul, Peerayuht Phuangphung, Mongkol Uiprasertkul, Sakda Sathirareuangchai, Chompunuch Boonarkart, Khwansiri Ninpan, Ornpreya Suptawiwat
Jazyk: angličtina
Předmět:
0301 basic medicine
Gene isoform
Adult
Male
alpha Karyopherins
animal structures
030106 microbiology
Short Report
Adaptation
Biological
Biology
medicine.disease_cause
Importin-α1 isoform
Human nasal mucosa
Real-Time Polymerase Chain Reaction
Virus Replication
environment and public health
Virus
03 medical and health sciences
Young Adult
Virology
medicine
Influenza A virus
Humans
Protein Isoforms
RNA
Messenger
Selection
Genetic
Importin-α3 isoform
Lung
Host cell nucleus
Importin-α7 isoform
Reverse Transcriptase Polymerase Chain Reaction
Human respiratory tract
Human lung cells
Alpha Karyopherins
Middle Aged
Influenza A virus subtype H5N1
Nasal Mucosa
030104 developmental biology
medicine.anatomical_structure
Infectious Diseases
Viral replication
embryonic structures
Interspecies barrier
Female
Respiratory tract
Zdroj: Virology Journal
ISSN: 1743-422X
DOI: 10.1186/s12985-016-0546-y
Popis: Background Transportation into the host cell nucleus is crucial for replication and transcription of influenza virus. The classical nuclear import is regulated by specific cellular factor, importin-α. Seven isoforms of importin-α have been identified in human. The preference of importin-α3 of avian influenza virus and -α7 isoform of human strains during replication in human cells was previously identified. In addition, both avian and human influenza viruses were shown to use importin-α1 isoform for their replication. Finding The mRNA levels of importin-α1, −α3, and –α7 isoforms in human respiratory tract was determined by real-time RT-PCR. The results indicate that mRNA level of importin-α7 was significantly higher than that of importin-α1 (p-value
Databáze: OpenAIRE
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