Covalent immobilization of hLf1-11 peptide on a titanium surface reduces bacterial adhesion and biofilm formation

Autor: Ciro Pérez-Giraldo, José María Manero, Daniel Rodríguez, Maria C. Fernández-Calderón, Fernando Albericio, Carlos Mas-Moruno, Maria Godoy-Gallardo, Francisco Javier Gil
Přispěvatelé: Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica, Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
Rok vydání: 2014
Předmět:
GOLD SURFACES
Peptide
HUMAN LACTOFERRIN
Biochemistry
Bacterial Adhesion
Anti-Infective Agents
Coated Materials
Biocompatible
Materials Testing
Lactoferrin peptide
Titanium
chemistry.chemical_classification
Antiinfective agent
biology
THIOL
General Medicine
Adhesion
Antimicrobial
ESCHERICHIA-COLI
Silanization
Pèptids
Antimicrobial peptide
Protein Binding
Biotechnology
Materials science
Cell Survival
Surface Properties
Antimicrobial peptides
Biomedical Engineering
Bacterial adhesion
Biofunctionalization
Enginyeria dels materials [Àrees temàtiques de la UPC]
CELL-ADHESIVE
Microbiology
Biomaterials
Molecular Biology
CANDIDA-ALBICANS
Biofilm
IN-VITRO
Titani
ANTIMICROBIAL PEPTIDES
biology.organism_classification
IMPLANTS
Peptide Fragments
Lactoferrin
Streptococcus sanguinis
chemistry
Biofilms
RAY PHOTOELECTRON-SPECTROSCOPY
Adsorption
Peptides
Zdroj: Recercat. Dipósit de la Recerca de Catalunya
Universitat Jaume I
UPCommons. Portal del coneixement obert de la UPC
Universitat Politècnica de Catalunya (UPC)
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2014.03.026
Popis: Bacterial infection represents a major cause of implant failure in dentistry. A common approach to overcoming this issue and treating pen-implant infection consists in the use of antibiotics. However, the rise of multidrug-resistant bacteria poses serious concerns to this strategy. A promising alternative is the use of antimicrobial peptides due to their broad-spectrum activity against bacteria and reduced bacterial resistance responses. The aim of the present study was to determine the in vitro antibacterial activity of the human lactoferrin-derived peptide hLf1-11 anchored to titanium surfaces. To this end, titanium samples were functionalized with the hLf1-11 peptide either by silanization methods or physical adsorption. X-ray photoelectron spectroscopy analyses confirmed the successful covalent attachment of the hLf1-11 peptide onto titanium surfaces. Lactate dehydrogenase assay determined that hLf1-11 peptide did not affect fibroblast viability. An outstanding reduction in the adhesion and early stages of biofilm formation of Streptococcus sanguinis and Lactobacillus salivarius was observed on the biofunctionalized surfaces compared to control non-treated samples. Furthermore, samples coated with the hLf1-11 peptide inhibited the early stages of bacterial growth. Thus, this strategy holds great potential to develop antimicrobial biomaterials for dental applications. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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