Distinct effects of thrombospondin-1 and CISP/thrombospondin-2 on adrenocortical cell spreading
| Autor: | B. Lafeuillade, Jean-Jacques Feige, Sylvie Pellerin, Edmond M. Chambaz |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Thrombospondin-2 Biochemistry Cell Line Endocrinology Cell Movement In vivo Internal medicine Thrombospondin 1 Cell Adhesion medicine Animals Cell adhesion Molecular Biology Thrombospondin Membrane Glycoproteins biology Adrenal cortex Calcium-Binding Proteins Molecular biology In vitro Culture Media Fibronectins Fibronectin Blood medicine.anatomical_structure Adrenal Cortex biology.protein Cattle Thrombospondins Cell Adhesion Molecules Plastics |
| Zdroj: | Molecular and Cellular Endocrinology. 106:181-186 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/0303-7207(94)90201-1 |
| Popis: | Corticotropin-induced secreted protein (CISP) is a trimeric protein secreted by bovine adrenocortical cells in response to ACTH, that is likely to represent the bovine form of thrombospondin-2 (TSP2). This study was aimed at delineating the respective effects of CISP/TSP2 and TSP1 (thrombospondin-1) on adrenocortical cell attachment and spreading. TSP1 and CISP/TSP2 were found to slightly reduce the attachment of adrenocortical cells to plastic in the presence of serum but exhibited a pronounced differential effect on cell spreading. CISP/TSP2 inhibited adrenocortical cell spreading in a dose-dependent manner (maximal effect with 40 micrograms/ml) whereas TSP1 (up to 100 micrograms/ml) did not influence this process. The inhibition of spreading was observed whether plates were coated with CISP/TSP2 alone or with a mixture of CISP/TSP2 and fibronectin. We suggest that the inhibition of in vitro adrenocortical cell spreading by CISP/TSP2 is indicative of an implication of this protein in the migration of adrenocortical cells in vivo. |
| Databáze: | OpenAIRE |
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