Proteomic Analysis of Urothelium of Rats with Detrusor Overactivity Induced by Bladder Outlet Obstruction

Autor: Sung Ho Yun, Jae Sung Lim, Yu Kyung Tak, Gun-Hwa Kim, Joohyun Park, Seung Il Kim, Chi-Won Choi, Sang-Yeop Lee, Hyo Kyun Chung, Edmond Changkyun Park, Ju Hyun Shin, Koon Soon Kim, Minji Lee, Yong Gil Na
Rok vydání: 2018
Předmět:
Proteomics
0301 basic medicine
medicine.medical_specialty
Proteome
Urinary system
Urinary Bladder
030232 urology & nephrology
Urology
urologic and male genital diseases
Biochemistry
Analytical Chemistry
Bladder Urothelium
Rats
Sprague-Dawley
Pathogenesis
03 medical and health sciences
Bladder outlet obstruction
0302 clinical medicine
medicine
Animals
Nocturia
Protein Interaction Maps
Urothelium
Molecular Biology
Urinary bladder
Urinary Bladder
Overactive
business.industry
Research
Reproducibility of Results
Molecular Sequence Annotation
Organ Size
medicine.disease
humanities
female genital diseases and pregnancy complications
Up-Regulation
Urinary Bladder Neck Obstruction
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
Overactive bladder
Female
medicine.symptom
business
Biomarkers
Signal Transduction
Zdroj: Molecular & Cellular Proteomics. 17:948-960
ISSN: 1535-9476
DOI: 10.1074/mcp.ra117.000290
Popis: Overactive bladder (OAB) syndrome is a condition that has four symptoms: urgency, urinary frequency, nocturia, and urge incontinence and negatively affects a patient's life. Recently, it is considered that the urinary bladder urothelium is closely linked to pathogenesis of OAB. However, the mechanisms of pathogenesis of OAB at the molecular level remain poorly understood, mainly because of lack of modern molecular analysis. The goal of this study is to identify a potential target protein that could act as a predictive factor for effective diagnosis and aid in the development of therapeutic strategies for the treatment of OAB syndrome. We produced OAB in a rat model and performed the first proteomic analysis on the mucosal layer (urothelium) of the bladders of sham control and OAB rats. The resulting data revealed the differential expression of 355 proteins in the bladder urothelium of OAB rats compared with sham subjects. Signaling pathway analysis revealed that the differentially expressed proteins were mainly involved in the inflammatory response and apoptosis. Our findings suggest a new target for accurate diagnosis of OAB that can provide essential information for the development of drug treatment strategies as well as establish criteria for screening patients in the clinical environment.
Databáze: OpenAIRE
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