Recombinant murine steel factor stimulates in vitro production of granulocyte-macrophage progenitor cells
| Autor: | S. D. Lyman, Eisenman J, Teepe M, D Foxworthe, Douglas E. Williams, Anderson D |
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| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_treatment
Population Stem cell factor Biology Granulocyte-Macrophage Progenitor Cells Hematopoietic Cell Growth Factors Biochemistry Mice medicine Animals Progenitor cell education Molecular Biology education.field_of_study Stem Cell Factor Growth factor Macrophages Cell Biology Molecular biology Mice Mutant Strains Recombinant Proteins Hematopoiesis Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Immunology Bone marrow Stem cell Granulocytes |
| Zdroj: | Journal of cellular biochemistry. 50(3) |
| ISSN: | 0730-2312 |
| Popis: | The ability of murine Steel factor to promote the in vitro production of granulocyte-macrophage progenitor cells (CFU-GM) was examined in short-term liquid cultures. Bone marrow from C57BL/6J or Sl/Sld mice was placed in culture for seven days with either Steel factor alone or in the presence of IL-3. CFU-GM responsive to GM-CSF, IL-3, and CSF-1 were measured in the input population and again after 3 or 7 days in culture. Steel factor alone increased the number of all CUF-GM types as early as 3 days after culture initiation, with further increases at day 7. This effect was protentiated by the addition of IL-3. Production of CFU-GM by C57BL/6J or Sl/Sld marrow was comparable except for enhanced production of CSF-1 responsive progenitors by Sl/Sld marrow. A recombinant Sld protein was also shown to be equivalent to the wild-type protein in its capacity to promote CFU-GM production from normal bone marrow. © 1992 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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