Metformin Reverts Deleterious Effects of Advanced Glycation End-Products (AGEs) on Osteoblastic Cells
| Autor: | Ana María Cortizo, Liliana Bruzzone, León Schurman, Verónica Arnol, Claudia Sedlinsky, Antonio Desmond McCarthy, María Virginia Gangoiti |
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| Rok vydání: | 2008 |
| Předmět: |
Glycation End Products
Advanced medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism Cellular differentiation Bone Neoplasms Cell Line RAGE (receptor) chemistry.chemical_compound Endocrinology Glycation Cell Line Tumor Internal medicine Internal Medicine medicine Animals Cells Cultured Osteosarcoma Osteoblasts business.industry nutritional and metabolic diseases Cell Differentiation Serum Albumin Bovine Osteoblast General Medicine Metformin Rats Kinetics medicine.anatomical_structure chemistry Cell culture Advanced glycation end-product Alkaline phosphatase business medicine.drug |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 116:333-340 |
| ISSN: | 1439-3646 0947-7349 |
| Popis: | Advanced glycation endproducts (AGEs) are implicated in the complications of diabetes and ageing, affecting several tissues, including bone. Metformin, an insulin-sensitizer drug, reduces the risk of life-threatening macrovascular complications. We have evaluated the hypothesis that metformin can abrogate AGE-induced deleterious effects in osteoblastic cells in culture. In two osteoblast-like cell lines (UMR106 and MC3T3E1), AGE-modified albumin induced cell death, caspase-3 activity, altered intracellular oxidative stress and inhibited alkaline phosphatase activity. Metformin-treatment of osteoblastic cells prevented these AGE-induced alterations. We also assessed the expression of AGE receptors as a possible mechanism by which metformin could modulate the action of AGEs. AGEs-treatment of osteoblast-like cells enhanced RAGE protein expression, and this up-regulation was prevented in the presence of metformin. Although the precise mechanisms involved in metformin signaling are still elusive, our data implicate the AGE-RAGE interaction in the modulation of growth and differentiation of osteoblastic cells. |
| Databáze: | OpenAIRE |
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