Changes in STEC and bacterial communities during enrichment of manufacturing beef in selective and non-selective media
| Autor: | Robert S. Barlow, Joshua T. Ravensdale, Ranil Coorey, Gary A. Dykes, Sanga Kang |
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| Rok vydání: | 2021 |
| Předmět: |
Meat
Population medicine.disease_cause Microbiology 03 medical and health sciences fluids and secretions Clostridium Escherichia medicine Animals Food science education Escherichia coli 030304 developmental biology 0303 health sciences education.field_of_study Shiga-Toxigenic Escherichia coli biology 030306 microbiology Chemistry Ribosomal RNA bacterial infections and mycoses biology.organism_classification Culture Media Food Microbiology bacteria Cattle Food Science |
| Zdroj: | Food Microbiology. 96:103711 |
| ISSN: | 0740-0020 |
| DOI: | 10.1016/j.fm.2020.103711 |
| Popis: | Detection and isolation of Shiga toxin-producing Escherichia coli (STEC) from manufacturing beef is challenging and it may be affected by microbial changes during enrichment. This study was designed to understand population changes during enrichment of beef from an integrated (Samples A and B) and a fragmented (Samples C and D) abattoir. The samples were enriched in buffered peptone water (BPW), Assurance GDS MPX top 7 STEC mEHEC®, BAX® E. coli O157:H7 MP and PDX-STEC media then were processed for 16 S rRNA sequencing. Escherichia dominated Sample B enrichment broths regardless of the media used (71.6–97.9%) but only in mEHEC broth (79.6%) of Sample A. Escherichia was dominant in Sample C in mEHEC (95.2%) and PDX-STEC (99.2%) broths but less in BPW (58.5%) and MP (64.9%) broths. In Sample D, Clostridium dominated in mEHEC (65.5%), MP (80.2%) and PDX-STEC (90.6%) broths. O157 STEC was isolated from Sample C only. The study suggested that MP may not be as effective as mEHEC and PDX-STEC and that Clostridium could interfere with enrichment of Escherichia. Understanding the ecological changes during enrichment provides meaningful insight to optimising the enrichment protocol for STEC and subsequently enhance the efficiency of STEC detection. |
| Databáze: | OpenAIRE |
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