Capillary zone electrophoresis of bacterial extracellular vesicles: A proof of concept
| Autor: | Bartosz Wielgomas, Michalina Zalewska, Martyna Piotrowska, Laura Sola, Bogdan Lewczuk, Krzysztof Waleron, Krzesimir Ciura, Paulina Sawicka, Szymon Dziomba, Bruna Correia, Marta Dawid, Joanna Kasprzyk, Wojciech Piekoszewski |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Pectobacterium 010402 general chemistry Mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry law.invention Extracellular Vesicles Capillary electrophoresis law Filtration Differential centrifugation Chromatography biology Chemistry Vesicle 010401 analytical chemistry Organic Chemistry Electrophoresis Capillary General Medicine biology.organism_classification Enterobacteriaceae 0104 chemical sciences Transmission electron microscopy Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Capillary electrophoresisExtracellular vesiclesMass spectrometryOuter membrane vesiclesPectobacteriumSoft rot bacteria Biomarkers |
| Zdroj: | Journal of chromatography A (Online) 1621 (2020). doi:10.1016/j.chroma.2020.461047 info:cnr-pdr/source/autori:Piotrowska M.; Ciura K.; Zalewska M.; Dawid M.; Correia B.; Sawicka P.; Lewczuk B.; Kasprzyk J.; Sola L.; Piekoszewski W.; Wielgomas B.; Waleron K.; Dziomba S./titolo:Capillary zone electrophoresis of bacterial extracellular vesicles: A proof of concept/doi:10.1016%2Fj.chroma.2020.461047/rivista:Journal of chromatography A (Online)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:1621 |
| Popis: | The extracellular vesicles (EVs) released by plant pathogens of the Pectobacterium genus were investigated. The isolates were obtained using differential centrifugation followed by filtration and were characterized in terms of total protein content and particle size distribution. The transmission electron microscopy (TEM) analysis revealed the presence of two morphologically differentiated subpopulations of vesicles in the obtained isolates. The proteomic analysis using matrix-assisted laser desorption ionization mass spectrometry with time of flight detector (MALDI-TOF/TOF-MS) enabled to identify 62 proteomic markers commonly found in EVs of Gram-negative rods from the Enterobacteriaceae family. Capillary electrophoresis (CE) was proposed as a novel tool for the characterization of EVs. The method allowed for automated and fast ( |
| Databáze: | OpenAIRE |
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