Effects of Opiates on High-Affinity Ca2+,Mg2+-ATPase in Brain Membrane Subfractions
| Autor: | David H. Ross, N P Pillai |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Narcotics
Calcium-Transporting ATPases (+)-Naloxone Pharmacology Biochemistry Naloxone Hydrochloride Cellular and Molecular Neuroscience medicine Animals Membranes Morphine biology Naloxone Chemistry Antagonist Brain Biological membrane Enzyme assay Rats Kinetics biology.protein Ca(2+) Mg(2+)-ATPase Opiate Intracellular medicine.drug |
| Zdroj: | Journal of Neurochemistry. 47:1642-1646 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1986.tb00807.x |
| Popis: | The effect of a single administration of morphine sulfate (15 mg/kg, s.c. or 30 mg/kg, i.p., 30 min) on Ca2+-stimulated Mg2+-dependent ATPase activity was investigated in synaptosomal plasma membranes (SPM) prepared from rat cortex. Morphine produced a significant decrease in Ca2+,Mg2+-ATPase activity in synaptosomal fractions (SPM 1 + 2) known to contain a high density of opiate receptors and calmodulin-dependent Ca2+,Mg2+-ATPase. However, in another subpopulation (SPM 3) that contains fewer opiate receptors and less enzyme activity, no such decrease in the enzyme activity was observed after the opiate administration. The decrease in Ca2+,Mg2+-ATPase activity seen in SPM 1 + 2 was specifically antagonized by the opiate antagonist naloxone hydrochloride (2 mg/kg, s.c.) when given 15 min before morphine administration. Mg2+-ATPase was not altered either by morphine or by a naloxone-morphine combination. These findings give further evidence for the role of intracellular Ca2+ in mediating many of the acute effects of opiates. |
| Databáze: | OpenAIRE |
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