Host immune response to infectious bronchitis virus Q1 in two commercial broiler chicken lines
Autor: | Kannan Ganapathy, Basim Manswr, Anne Forrester, Christopher Ball, Julian Chantrey |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
medicine.medical_specialty
animal structures 040301 veterinary sciences Infectious bronchitis virus Enzyme-Linked Immunosorbent Assay Microbiology 0403 veterinary science Pathogenesis 03 medical and health sciences Immune system medicine Animals Poultry Diseases 030304 developmental biology 0303 health sciences General Veterinary biology Immunity Broiler Proventriculus 04 agricultural and veterinary sciences Viral Load biology.protein Histopathology Antibody Coronavirus Infections Chickens Viral load |
Zdroj: | RESEARCH IN VETERINARY SCIENCE |
Popis: | This study investigated the pathogenesis of infectious bronchitis virus (Gammacoronavirus) strain Q1 in two commercial broiler chicken lines, and the host immune response to infection. Chicks from each line were grouped into either infected or control. Following Q1 infection at day-old, fast (Line-A) and slow (Line-B) growing chicks were monitored for clinical signs and body weights. At 3, 7, 9, 14, 21 and 28 days post infection (dpi), five birds were humanely euthanised, and trachea, kidney and proventriculus tissues were collected for quantitative RT-PCR and histopathology. Blood was collected weekly to determine IBV-specific ELISA antibody titres. Q1 infection significantly reduced the body weights of Line-A chicks at 14 and 21 dpi, but there were no significant differences in Line-B. Through qRT-PCR, significantly higher viral loads were found in the trachea, proventriculus and kidney tissues of Line-A chicks at 7–9 dpi. At day-old and at 28 dpi, the mean antibody titre in Line-B was notably higher than Line-A. Significant IFN-α mRNA expression was noted in the trachea and kidneys of Line-A, whereas no change occurred in Line-B. Chicks in Line-B, compared to those in Line-A, demonstrated a tissue-dependent increase of IFN-β, TLR3, IL-1β and IL-6 and LITAF gene transcription responses to IBV Q1. It appears that the level of maternal antibodies, growth rates, and other inherent host genetic factors could have influenced the differences in viral loads and immune responses. |
Databáze: | OpenAIRE |
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