Preeclampsia: inflammatory signature of decidual cells in early manifestation of disease
| Autor: | A.V. Lokhonina, K. Midiber, Timur Fatkhudinov, Polina Vishnyakova, L. Mikhaleva, Maria Nikitina, Andrey Elchaninov, Gennadiy T. Sukhikh, N. Loginova, A. Potapova, Z.S. Khodzhaeva, K. Muminova, A. Pyregov, Anastasiya S. Poltavets, V. Vtorushina |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
0301 basic medicine Inflammation Preeclampsia Andrology 03 medical and health sciences 0302 clinical medicine Pre-Eclampsia Pregnancy Placenta Decidua medicine Humans Decidual cells Endothelial dysfunction reproductive and urinary physiology Fetus 030219 obstetrics & reproductive medicine business.industry Macrophages Obstetrics and Gynecology Flow Cytometry medicine.disease female genital diseases and pregnancy complications Phenotype 030104 developmental biology medicine.anatomical_structure Reproductive Medicine embryonic structures Cytokines Female medicine.symptom business CD163 Developmental Biology |
| Zdroj: | Placenta. 104:277-283 |
| ISSN: | 0143-4004 |
| DOI: | 10.1016/j.placenta.2021.01.011 |
| Popis: | Introduction Preeclampsia is a pregnancy-specific complication characterized by hypertension in combination with proteinuria and/or various manifestations of multiple organ failure. It is believed that etiology of preeclampsia lies in dysfunction of the placenta and disorder of the maternal-fetal interactions. In preeclampsia decidual membrane, the maternal part of the placenta which normally supports immunological tolerance of the maternal organism to the semi-allogeneic fetus, becomes a site of inflammation. Methods The aim of our study was to characterize the phenotype of decidual macrophages and plasma profiles in patients with late- and early-onset preeclampsia as compared with controls (n = 43). Decidual cells were obtained by enzymatic digestion method and characterized by flow cytometry analysis, real-time PCR, bioinformatics analysis, immunohistochemistry, and Western blot. Plasma samples were analyzed by multiplex assay. Results The number of inflammation-associated CD86+ and CX3CR1+ cells was significantly higher in the early-onset preeclampsia while the portion of CD163+ cells was significantly higher among studied groups. We observed significant increase of endothelin-1 gene expression and a significant decrease in eNOS and GNB3 expression and TGFβ relative protein level in decidual cells of the early-onset preeclampsia samples. We also revealed elevation of pro- and anti-inflammatory cytokines in plasma of preeclampsia groups. Discussion Our findings reflect profound early-onset preeclampsia-associated alterations in the decidua and emphasize the importance of the decidua as a link in the development of preeclampsia. |
| Databáze: | OpenAIRE |
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