A new method for determination of inorganic phosphorus in serum without deproteinization

Autor:	Abdelbaset Anwer-El-Aaser, Mahmoud Mohamed El-Merzabani, Nadia Iskandar Zakhary
Rok vydání:	1977
Předmět:	Glycerol Molybdenum Chromatography Formates Chemistry Formic acid education Biochemistry (medical) Clinical Biochemistry General Medicine Phosphates chemistry.chemical_compound Solubilization Present method Methods Humans Colorimetry Trichloroacetic acid Inorganic phosphorus
Zdroj:	Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie. 15(12)
ISSN:	0340-076X
Popis:	A simple method was developed for the determination of inorganic phosphorus in serum without deproteinization. The method is based on the use of formic acid as protein solubilizer and glycerol as stabilizer for the assay system. The optimal conditions for colour development were determined. The results obtained with the new method correlate well with those obtained after deproteinization of serum with trichloroacetic acid. The present method could be fully mechanized, and its application to the determination of serum phosphatases is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a8125be3a74e34a0c73d4fc9188478b https://pubmed.ncbi.nlm.nih.gov/604424 Zobrazit plný text záznamu