A novel bacterial transcription cycle involving sigma 54
Autor: | Yin Tintut, Jay D. Gralla, Jonathan T. Wang |
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Rok vydání: | 1995 |
Předmět: |
DNA
Bacterial Time Factors Transcription Genetic DNA Footprinting DNA Single-Stranded DNA footprinting Sigma Factor chemistry.chemical_compound Bacterial Proteins Potassium Permanganate Bacterial transcription Sigma factor Transcription (biology) RNA polymerase Escherichia coli Genetics Polymerase Models Genetic biology Escherichia coli Proteins fungi Promoter DNA-Directed RNA Polymerases Cell biology DNA-Binding Proteins chemistry Coding strand biology.protein RNA Polymerase Sigma 54 Protein Binding Developmental Biology |
Zdroj: | Genes & Development. 9:2305-2313 |
ISSN: | 1549-5477 0890-9369 |
DOI: | 10.1101/gad.9.18.2305 |
Popis: | sigma 54 is the promoter recognition subunit of the form of bacterial RNA polymerase that transcribes from promoters with enhancer elements. DNase footprinting experiments show that sigma 54 is attached selectively to the template strand, which must be single-stranded for transcription initiation. sigma 54 remains bound at the promoter after core polymerase begins elongation, in contrast to the well-established sigma 70-holoenzyme transcription cycle. Permanganate footprinting experiments show that the bound sigma 54 and the elongating core RNA polymerase downstream of it are each associated with a single-strand DNA region. Template commitment assays show that the promoter-bound sigma 54 must be reconfigured before reinitiation of transcription can occur. This unexpected pathway raises interesting possibilities for transcriptional regulation, especially with regard to control at the level of reinitiation. |
Databáze: | OpenAIRE |
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