Apoptosis: a mechanism contributing to remodeling of skeletal muscle in response to hindlimb unweighting
| Autor: | Jon K. Linderman, V. R. Mukku, David L. Allen, A. J. Bigbee, R. E. Grindeland, Roland R. Roy, V. R. Edgerton |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Pathology Time Factors Physiology medicine.medical_treatment Apoptosis Hindlimb Biology Rats Sprague-Dawley Weight-Bearing Atrophy Physical Conditioning Animal Internal medicine medicine Animals Insulin-Like Growth Factor I Muscle Skeletal Soleus muscle Growth factor Skeletal muscle Cell Biology medicine.disease Muscle atrophy Rats Endocrinology medicine.anatomical_structure Terminal deoxynucleotidyl transferase Female medicine.symptom |
| Zdroj: | American Journal of Physiology-Cell Physiology. 273:C579-C587 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1997.273.2.c579 |
| Popis: | The role of apoptosis in the elimination of myonuclei during hindlimb unloading-induced atrophy and the inhibition of apoptosis in the prevention of muscle atrophy were examined. The number of nuclei demonstrating double-stranded DNA fragmentation seen by terminal deoxynucleotidyl transferase (TDT) histochemical staining, an indicator of apoptosis, was significantly increased after 14 days of suspension. Double staining with TDT and antilaminin immunohistochemistry revealed that some TDT-positive nuclei were within the fiber lamina and were most likely myonuclei. The number of fibers containing morphologically abnormal nuclei was also significantly greater in suspended compared with control rats. Combined treatment with growth hormone and insulin-like growth factor I (GH/ IGF-I) and resistance exercise attenuated the increase in TDT-positive nuclei (approximately 26%, P > 0.05) and significantly decreased the number of fibers with morphologically abnormal nuclei. The data suggest that 1) “programmed nuclear death” contributes to the elimination of myonuclei and/or satellite cells from atrophying fibers, and 2) GH/IGF-I administration plus muscle loading ameliorates the apoptosis associated with hindlimb unloading. |
| Databáze: | OpenAIRE |
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