COMPARATIVE EFFECTS OF HERPES SIMPLEX VIRUS TYPES 1 AND 2 IN ORGANOTYPIC CULTURES OF MOUSE DORSAL ROOT GANGLION
Autor: | Teresita S. Elizan, Jerome Schwartz, William O. Whetsell |
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Rok vydání: | 1977 |
Předmět: |
Simplexvirus
Time Factors food.ingredient viruses Biology Virus Replication medicine.disease_cause Virus Pathology and Forensic Medicine Mice Cellular and Molecular Neuroscience Myelin Organ Culture Techniques food Dorsal root ganglion Ganglia Spinal Virus latency medicine Animals Myelin Sheath Neurons Herpesviridae Infections General Medicine medicine.disease Virology Cell nucleus medicine.anatomical_structure Herpes simplex virus Neurology Viral replication Neurology (clinical) |
Zdroj: | Journal of Neuropathology and Experimental Neurology. 36:547-560 |
ISSN: | 0022-3069 |
Popis: | Mature organized cultures of mouse dorsal root ganglion (MDRG) were infected with herpes simplex virus, type 1 (HSV 1) and type 2 (HSV 2). Onset of infectious virus production occurred faster and reached higher levels in HSV 2-infected cultures. Neurons, supporting cells and myelin were affected in both types of infection, but morphological changes occurred significantly earlier and more dramatically with the type 2 infection. The pattern of myelin changes was distinctly different in the two types of infection. Within 20 hours post infection nerve cells infected with HSV 2 developed several types of intranuclear inclusions consisting of membranes and filaments; no such neuronal inclusions were seen with HSV 1 infection. HSV 2 infection showed frequent, large, membranous inclusions in supporting cell nuclei whereas, only rare, small inclusions of this type were seen in supporting cells infected with HSV 1. The observations demonstrate that the two virus types produced different virus replication patterns and different morphologic changes in long term cultures of MDRG. There appears to be a differential response of neurons and non-neuronal elements to the virus in this tissue substrate. Viral latency was not induced in this system by direct inoculation of the virus under the conditions described. |
Databáze: | OpenAIRE |
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