Engineered Nanostructures of Haptens Lead to Unexpected Formation of Membrane Nanotubes Connecting Rat Basophilic Leukemia Cells
| Autor: | Ted A. Laurence, Ying X. Liu, Kang Hsin Wang, Fu-Tong Liu, Yang Liu, Shailise S. Ross, Huan Yuan Chen, Gang-yu Liu, Jie Ren Li |
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| Rok vydání: | 2015 |
| Předmět: |
CCR1
Cell General Physics and Astronomy particle lithography mast cells Bioengineering Immunoglobulin E Cell Membrane Structures Article Cell Line Chemokine receptor Rare Diseases Antigen Cell Line Tumor medicine Animals rat basophilic leukemia (RBL) cells Nanotechnology 2.1 Biological and endogenous factors General Materials Science Aetiology Nanoscience & Nanotechnology Receptor Macrophage inflammatory protein Cancer Tumor atomic force microscopy biology Chemistry haptens Inflammatory and immune system General Engineering hemic and immune systems Hematology Cell biology Basophils Rats Nanostructures medicine.anatomical_structure biology.protein Hapten Haptens scanning electron microscopy membrane nanotubes |
| Zdroj: | ACS nano, vol 9, iss 7 |
| Popis: | A recent finding reports that co-stimulation of the high-affinity immunoglobulin E (IgE) receptor (FcεRI) and the chemokine receptor 1 (CCR1) triggered formation of membrane nanotubes among bone-marrow-derived mast cells. The co-stimulation was attained using corresponding ligands: IgE binding antigen and macrophage inflammatory protein 1α (MIP1 α), respectively. However, this approach failed to trigger formation of nanotubes among rat basophilic leukemia (RBL) cells due to the lack of CCR1 on the cell surface (Int. Immunol. 2010, 22 (2), 113-128). RBL cells are frequently used as a model for mast cells and are best known for antibody-mediated activation via FcεRI. This work reports the successful formation of membrane nanotubes among RBLs using only one stimulus, a hapten of 2,4-dinitrophenyl (DNP) molecules, which are presented as nanostructures with our designed spatial arrangements. This observation underlines the significance of the local presentation of ligands in the context of impacting the cellular signaling cascades. In the case of RBL, certain DNP nanostructures suppress antigen-induced degranulation and facilitate the rearrangement of the cytoskeleton to form nanotubes. These results demonstrate an important scientific concept; engineered nanostructures enable cellular signaling cascades, where current technologies encounter great difficulties. More importantly, nanotechnology offers a new platform to selectively activate and/or inhibit desired cellular signaling cascades. |
| Databáze: | OpenAIRE |
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