Developmental Changes in Cyclooxygenase mRNA Expression in the Gastric Mucosa of Rats
| Autor: | Akio Hikima, Tetsushi Ogawa, Akihiro Morikawa, Hiroaki Kaneko, Takeshi Tomomasa, Masahiko Tabata |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Aging
medicine.medical_specialty Lipopolysaccharide Polymerase Chain Reaction Isozyme Gene Expression Regulation Enzymologic Prostaglandin-endoperoxide synthase 2 chemistry.chemical_compound Internal medicine medicine Gastric mucosa Animals RNA Messenger Rats Wistar Regulation of gene expression Messenger RNA biology Stomach Age Factors Gastroenterology Membrane Proteins Rats Isoenzymes medicine.anatomical_structure Endocrinology chemistry Cyclooxygenase 2 Gastric Mucosa Prostaglandin-Endoperoxide Synthases Pediatrics Perinatology and Child Health Cyclooxygenase 1 biology.protein Cyclooxygenase |
| Zdroj: | Journal of Pediatric Gastroenterology and Nutrition. 34:169-173 |
| ISSN: | 0277-2116 |
| DOI: | 10.1097/00005176-200202000-00012 |
| Popis: | Background In newborn rats, gastric mucosa is more susceptible to various damaging agents and recovers from injury more quickly than in older animals. To determine whether metabolism of prostaglandins is responsible for this mucosal protective mechanism in developing rats, we studied cyclooxygenase (COX) mRNA expression in the mucosa using quantitative real-time polymerase chain reaction (PRC). Methods Cyclooxygenase-1 and COX-2 mRNA was extracted from the gastric mucosa of rats of various ages and quantitatively analyzed using real-time PCR with dual-labeled fluorogenic probes. The copy numbers of cDNA for COX-1 and COX-2 were standardized to glyceraldehyde-3-phosphate dehydrogenase from the same sample. Results Cyclooxygenase-1 mRNA expression was lowest in 1-week-old rats and highest in 4-week-old rats. Mucosal damage produced by 150 mmol/L HCl and 60% ethyl alcohol did not increase COX-1 mRNA expression in any age group. Cyclooxygenase-2 mRNA expression increased significantly with age. Mucosal injury increased COX-2 mRNA in each age group, especially in 1-week-old rats. Intraperitoneal lipopolysaccharide also increased COX-2 mRNA in both 1- and 4-week old rats. Conclusion The high level of COX-2 mRNA expression in the gastric mucosa of 1-week-old rats may be responsible for the physiologic characteristics of gastric mucosal defenses in this age group. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text