Evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using PVA-immobilized cell beads
| Autor: | Wen-Bin Yang, Sz-Chwun John Hwang, Jane-Yii Wu, Kuo-Cheng Chen |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Acinetobacter baumannii
Diffusion Batch reactor Kinetics Analytical chemistry Bioengineering Applied Microbiology and Biotechnology Polyvinyl alcohol Reaction rate chemistry.chemical_compound Biomass Phosphorylation Coloring Agents Comamonas testosteroni Chromatography Chemistry Proteins Cells Immobilized Biodegradation Thiele modulus Microspheres Aeromonas hydrophila Partition coefficient Polyvinyl Alcohol Microscopy Electron Scanning Azo Compounds Gels Mathematics Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 83:821-832 |
| ISSN: | 1097-0290 0006-3592 |
| Popis: | An immobilized mixed culture (Aeromonas hydrophila, Comamonas testosteroni, and Acinetobacter baumannii) was prepared by entrapment into phosphorylated polyvinyl alcohol (PVA) gel beads. The unsteady-state diffusion mechanism in a gel bead was applied to estimate the effective diffusion coefficients (De) and the partition coefficients (Kp) of azo dye. In addition, a simple method was developed to determine the intrinsic kinetic parameters of immobilized cells from observed reaction rates and the intrinsic kinetic parameters were then verified by fitting the experimental data into the reaction-diffusion model in a batch reactor running at a well-stirred state. The calculated effectiveness factor (ηcal) approached unity at Thiele modulus (Φ) < 0.3 (i.e., dp < 0.475 mm). The experimental effectiveness factor (ηexp) was in the range of 0.71–0.45 for a corresponding sphere diameter (dp) of 1.91 ± 0.16 to 4.43 ± 0.07 mm at an initial dye concentration of 200 mg/L. The results show that intraparticle diffusion resistance has a significant effect on the azo dye biodegradation rate. © 2003 Wiley Periodicals. Biotechnol Bioeng 83: 821–832, 2003. |
| Databáze: | OpenAIRE |
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