Unspecific binding of group B streptococcal cocytolysin (CAMP factor) to immunoglobulins and its possible role in pathogenicity
| Autor: | F J Fehrenbach, D Jürgens, B Sterzik |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
Myeloma protein Immunology Spleen CAMP test Streptococcus agalactiae Immunoenzyme Techniques Hemolysin Proteins Mice Bacterial Proteins Sepsis Streptococcal Infections Protein A/G medicine Animals Immunology and Allergy biology Histocytochemistry Lethal dose Antibodies Monoclonal Articles medicine.disease Molecular biology Hemolysis Immunoglobulin Fc Fragments medicine.anatomical_structure Immunoglobulin M Liver Polyclonal antibodies Immunoglobulin G biology.protein Binding Sites Antibody Antibody |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.165.3.720 |
| Popis: | The protein B of group B streptococci can bind in a nonimmune reaction to Ig of the IgG and IgM classes of various mammalian species (i.e., human, mouse, rabbit, and bovine). Protein B binding involves the Fc parts of both IgG and IgM molecules. Monoclonal or polyclonal IgG or IgM and the IgM-FC5 mu fragment of human myeloma protein combined with the protein B thereby inhibiting protein B-induced hemolysis in the CAMP reaction. The protein B/Ig complex can be dissociated with 1% Triton or guanidine-HCl (6 M). Mice infected intraperitoneally with sublethal doses of group B streptococci (GBS) and that received seven repeated intravenous injections of highly purified protein B during the first 9 h of infection developed fatal septicemia within 24 h with colony counts of up to 10(8) CFU/ml in the blood. Animals treated in the same way with either PBS or trypsinized protein B recovered. The protein B itself was not pathogenic when injected into healthy mice. Tissue sections of liver or spleen from mice infected with a lethal dose of GBS revealed the presence of protein B together with large numbers of cocci when stained by the peroxidase method using specific antibodies raised against purified protein B in the rabbit. |
| Databáze: | OpenAIRE |
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