A validation study of microscopy versus quantitative PCR for measuring Plasmodium falciparum parasitemia
| Autor: | Zuleima Pava, Louise Marquart, Tran Tinh Hien, Emma Ballard, Peter O'Rourke, Claire Y. T. Wang, Nguyen Thanh Tong, James S. McCarthy, Joel Tarning |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Validation study
lcsh:Arctic medicine. Tropical medicine Intraclass correlation lcsh:RC955-962 030231 tropical medicine Plasmodium falciparum Short Report PLASMODIUM FALCIPARUM PARASITEMIA Parasitemia Biology Andrology 03 medical and health sciences 0302 clinical medicine Microscopy parasitic diseases Validation medicine 030212 general & internal medicine Parasite density Public Health Environmental and Occupational Health biology.organism_classification medicine.disease 3. Good health qPCR Infectious Diseases Real-time polymerase chain reaction |
| Zdroj: | Tropical Medicine and Health Tropical Medicine and Health, Vol 47, Iss 1, Pp 1-4 (2019) |
| ISSN: | 1349-4147 1348-8945 |
| Popis: | Microscopy and 18S qPCR are the most common and field-friendly methods for quantifying malaria parasite density, and it is important that these methods can be interpreted as giving equivalent results. We compared results of quantitative measurement of Plasmodium falciparum parasitemia by microscopy and by 18S qPCR in a phase 2a study. Microscopy positive samples (n = 355; median 810 parasites/μL [IQR 40–10,471]) showed close agreement with 18S qPCR in mean log10/mL transformed parasitemia values by paired t test (difference 0.04, 95%CI − 0.01–0.10, p = 0.088). Excellent intraclass correlation (0.97) and no evidence of systematic or proportional differences by Passing–Bablok regression were observed. 18S qPCR appears to give equivalent parasitemia values to microscopy, which indicates 18S qPCR is an appropriate alternative method to quantify parasitemia in clinical trials. |
| Databáze: | OpenAIRE |
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