Nanometer-scale optical imaging of collagen fibers using gold nanoparticles
| Autor: | Christian Hellriegel, Bo Chen, Enrico Gratton, Laura C. Estrada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Materials science
(160.4236) Nanomaterials Nanotechnology 02 engineering and technology ocis:(170.0180) Microscopy Nanomaterials law.invention 03 medical and health sciences law ocis:(110.5125) Photoacoustics Microscopy Medicine and Health Sciences Physical Sciences and Mathematics Fiber (110.5125) Photoacoustics (170.0180) Microscopy 030304 developmental biology Photoacoustic effect 0303 health sciences Total internal reflection ocis:(160.4236) Nanomaterials ocis:(300.6280) Spectroscopy fluorescence and luminescence Life Sciences (300.6280) Spectroscopy fluorescence and luminescence 021001 nanoscience & nanotechnology Laser Atomic and Molecular Physics and Optics Colloidal gold Temporal resolution 0210 nano-technology Biotechnology |
| Zdroj: | Biomedical Optics Express Chen, Bo; Estrada, Laura C.; Hellriegel, Christian; & Gratton, Enrico. (2011). Nanometer-scale optical imaging of collagen fibers using gold nanoparticles. Biomedical Optics Express, 2(3), 511-519. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/1ft2k4x5 |
| ISSN: | 2156-7085 |
| Popis: | We describe 3D single particle tracking of gold nanoparticles (AuNPs) moving along collagen fibers in aqueous environment with two-photon excitation conditions. The photoacoustic effect at the collagen fiber caused by the irradiation with ultrashort, near-infrared laser pulses propels the particles adsorbed to the surface of the collagen fibers. We report the tracking of individual AuNPs in three dimensions with high spatial and temporal resolution, of few nanometers and milliseconds, respectively. Due to the emission signal caused by the interaction between the AuNPs and the weak chromophores in the collagen fiber, the trajectories of individual AuNPs reveal the fiber topography with nanometric resolution. The intensity along the trajectory shows that we are sensitive to the distribution of the weak chromophores on the fiber. |
| Databáze: | OpenAIRE |
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