Analytical sensitivity and clinical sensitivity of the three rapid antigen detection kits for detection of SARS-CoV-2 virus
| Autor: | Gannon C.K. Mak, Dominic Nc Tsang, Kitty K.Y. Wong, Edman Tk Lam, Nancy L.S. Chow, Rickjason C.W. Chan, Stephen S.Y. Lau, C.S. Lau |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
2019-20 coronavirus outbreak Time Factors Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology RT-PCR Sensitivity and Specificity Virus Article COVID-19 Serological Testing 03 medical and health sciences 0302 clinical medicine rapid antigen detection Antigen Limit of Detection Virology Nasopharynx Medicine Humans 030212 general & internal medicine Antigens Viral Detection limit 2019 novel coronavirus business.industry SARS-CoV-2 COVID-19 eye diseases Infectious Diseases COVID-19 Nucleic Acid Testing Reagent Kits Diagnostic business |
| Zdroj: | Journal of Clinical Virology |
| ISSN: | 1873-5967 1386-6532 |
| Popis: | Highlights • We compared analytical sensitivity and clinical sensitivity for the three commercially available rapid antigen detection (RAD) kits for detection of SARS-CoV-2 virus. • The three RAD kits varied 102 to 105 fold less sensitive than RT-PCR. • Clinical sensitivity of RAD kits ranged from 22.9% to 71.4% for detecting respiratory specimens from COVID-19 patients. • Understanding the clinical characteristics of different RAD kits can increase the likelihood of positive results. Background Numerous rapid antigen detection (RAD) kits for diagnosing COVID-19 patients are available in the market recently. Objective To compare analytical sensitivity and clinical sensitivity for the three commercially available RAD kits. Study Design Analytical sensitivity for the detection of SARS-CoV-2 virus was determined by limit of detection (LOD) using PCR as a reference method. Clinical sensitivity was evaluated by using respiratory specimens collected from confirmed COVID-19 patients. Results The LOD results showed that the three RAD kits varied 102 to 105 fold less sensitive than RT-PCR. Clinical sensitivity of RAD kits ranged from 22.9% to 71.4% for detecting specimens from COVID-19 patients. Conclusions Although RAD kits were less sensitive than RT-PCR, understanding the clinical characteristics of different RAD kits can guide us to obtain suitable specimens for testing. The likelihood of positive results for RAD kits will be higher. |
| Databáze: | OpenAIRE |
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