Priming of normal human neutrophils by recombinant human growth hormone
Autor: | Christian J. Wiedermann, Monika Niedermühlbichler, Hugo Beimpold, Dietmar Geissler, Herbert Braunsteiner |
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Rok vydání: | 1991 |
Předmět: |
medicine.medical_specialty
Neutrophils Nitroblue Tetrazolium Growth factor medicine.medical_treatment Neutrophile Priming (immunology) Motility hemic and immune systems Chemotaxis Hematology Biology Recombinant Proteins Respiratory burst Endocrinology Immune system Cell Movement Growth Hormone Internal medicine Cell Adhesion medicine Humans Chemotaxis assay |
Zdroj: | British Journal of Haematology. 78:19-22 |
ISSN: | 1365-2141 0007-1048 |
DOI: | 10.1111/j.1365-2141.1991.tb04376.x |
Popis: | Summary Growth hormone (GH) plays an important role in the development, maintenance and function of the immune system. Previous data has demonstrated that GH is also a newly defined macrophage-activating factor. Activation of polymorphonuclear neutrophils (PMN) by GH has not yet been examined. This paper presents studies demonstrating the effects of GH on the migratory behaviour and respiratory burst of PMN. In a modified Boyden chamber chemotaxis assay, GH did not stimulate PMN locomotion when added directly to the cells but potently inhibited formylpeptide-stimulated chemotaxis with effective concentrations in the picomolar range. The migration inhibition observed is known from studies on PMN priming-factors to be due to enhanced adhesiveness of PMN to artificial surfaces such as nitrocellulose, suggesting that GH stimulates PMN adhesiveness. Priming of PMN by GH was confirmed by direct demonstration of a stimulatory effect on reduction of nitroblue tetrazolium. These findings suggest that GH may be involved in the regulation of PMN functions. |
Databáze: | OpenAIRE |
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