Comparative evaluation of the indigenous microbial diversity vs. drilling fluid contaminants in the NEEM Greenland ice core
| Autor: | Jean E. Brenchley, Todd Sowers, Vanya Miteva, Caroline Burlingame |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Firmicutes
Earth science Genes Fungal Greenland Molecular Sequence Data Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Ascomycota Microbial ecology Ice core RNA Ribosomal 16S Drilling fluid Proteobacteria Environmental Microbiology Ice Cover 14. Life underwater Phylogeny 030304 developmental biology 0303 health sciences geography geography.geographical_feature_category Ecology biology 030306 microbiology Basidiomycota Ice Drilling Glacier Biodiversity DNA Contamination Contamination biology.organism_classification Actinobacteria Molecular Typing Greenland ice core project Genes Bacterial 13. Climate action human activities |
| Zdroj: | FEMS Microbiology Ecology. 89:238-256 |
| ISSN: | 0168-6496 |
| DOI: | 10.1111/1574-6941.12286 |
| Popis: | Demonstrating that the detected microbial diversity in nonaseptically drilled deep ice cores is truly indigenous is challenging because of potential contamination with exogenous microbial cells. The NEEM Greenland ice core project provided a first-time opportunity to determine the origin and extent of contamination throughout drilling. We performed multiple parallel cultivation and culture-independent analyses of five decontaminated ice core samples from different depths (100-2051 m), the drilling fluid and its components Estisol and Coasol, and the drilling chips collected during drilling. We created a collection of diverse bacterial and fungal isolates (84 from the drilling fluid and its components, 45 from decontaminated ice, and 66 from drilling chips). Their categorization as contaminants or intrinsic glacial ice microorganisms was based on several criteria, including phylogenetic analyses, genomic fingerprinting, phenotypic characteristics, and presence in drilling fluid, chips, and/or ice. Firmicutes and fungi comprised the dominant group of contaminants among isolates and cloned rRNA genes. Conversely, most Proteobacteria and Actinobacteria originating from the ice were identified as intrinsic. This study provides a database of potential contaminants useful for future studies of NEEM cores and can contribute toward developing standardized protocols for contamination detection and ensuring the authenticity of the microbial diversity in deep glacial ice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text