Changes in Osteoblast, Chondrocyte, and Adipocyte Lineages Mediate the Bone Anabolic Actions of PTH and Small Molecule GSK-3 Inhibitor

Autor:	Richard E. Higgs, Thomas A. Engler, Henry Uhlman Bryant, Ernst R. Dow, Mathias N'Cho, Bruce D. Gitter, Jude E. Onyia, Jianyong Shou, Trent R. Stewart, David L. Halladay, Tao Wei, T. John Martin, Yanfei L. Ma, Diane L. Sterchi, Amar Kumar, N.H. Kulkarni
Rok vydání:	2007
Předmět:	medicine.medical_specialty Time Factors Stromal cell Injections Subcutaneous Ovariectomy Gene Expression Parathyroid hormone Bone Marrow Cells Biology Models Biological Biochemistry Drug Administration Schedule Chondrocyte Rats Sprague-Dawley Glycogen Synthase Kinase 3 Chondrocytes Internal medicine Adipocytes medicine Animals Humans Cell Lineage Enzyme Inhibitors Molecular Biology Cells Cultured Oligonucleotide Array Sequence Analysis Osteoblasts Tibia Stem Cells Osteoblast Cell Biology Alkaline Phosphatase medicine.disease Chondrogenesis Peptide Fragments Rats Osteopenia Disease Models Animal Endocrinology medicine.anatomical_structure Parathyroid Hormone Adipogenesis Ovariectomized rat Female Stromal Cells Biomarkers hormones hormone substitutes and hormone antagonists
Zdroj:	Journal of Cellular Biochemistry. 102:1504-1518
ISSN:	1097-4644 0730-2312
Popis:	Parathyroid hormone (PTH) and glycogen synthase kinase-3 (GSK-3) inhibitor 603281-31-8, administered once daily increased bone formation in vivo. We investigated the molecular mechanisms of the anabolic responses of PTH and 603281-31-8 in rat osteopenia model. Female 6-month-old rats were ovariectomized (Ovx) and permitted to lose bone for 1 month, followed by treatment with PTH (1-38) at 10 microg/kg/day s.c. or 603281-31-8 at 3 mg/kg/day p.o. for 60 days. Twenty-four hours after the last treatment, RNA from distal femur metaphysis was subjected to gene expression analysis. Differentially expressed genes (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a6dfee062afef2e3f0ef7661e0c47e9 https://doi.org/10.1002/jcb.21374 Zobrazit plný text záznamu Plný text