Cytotoxicity and Epidermal Barrier Function Evaluation of Common Antiseptics for Clinical Use in an Artificial Autologous Skin Model

Autor: Trinidad Montero-Vilchez, Salvador Arias-Santiago, A. Fernández-González, Elena Pérez-Castejón, María I Quiñones-Vico
Přispěvatelé: [Quiñones-Vico,MI, Fernández-González,A, Pérez-Castejón,E, Arias-Santiago,S] Cell Production and Tissue Engineering Unit, Virgen de las Nieves University Hospital, Granada, Spain. [Quiñones-Vico,MI, Montero-Vílchez,T, Arias-Santiago,S] Biosanitary Institute of Granada (ibs. GRANADA), Granada, Spain. [Quiñones-Vico,MI, Arias-Santiago,S] Andalusian Network of Design and Translation of Advanced Therapies, Sevilla, Spain. [Quiñones-Vico,MI, Arias-Santiago,S] Dermatology Department, School of Medicine, University of Granada, Granada, Spain. [Montero-Vílchez,T, Arias-Santiago,S] Dermatology Department, Virgen de las Nieves University Hospital, Granada, Spain., The work of María I. Quiñones Vico was supported by a predoctoral fellowship (BOE 22/10/2019) from the Ministry of Science, Innovation and Universities of Spain and by a researchinitiation grant of the University of Granada. This study is part of her doctoral research in the Biomedicine’s program of University of Granada. This study was funded by the Carlos III Health Institute of Spain through the project PI13/02576 and PI17/02083 (co-funded by European Regional Development Fund 'A way to make Europe') and Andalusian Regional Government Financial (SAS PI-0458-2016).
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Cell viability
Antibiotics
Polyhexanide
lcsh:Medicine
wound healing
Piel artificial
Pharmacology
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Surgical Equipment::Artificial Organs::Skin
Artificial [Medical Subject Headings]
Antiinfecciosos locales
Supervivencia celular
Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]
030207 dermatology & venereal diseases
chemistry.chemical_compound
Bass (fish)
0302 clinical medicine
Medicina regenerativa
Antiseptic
Medicine
Analytical
Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Clinical Protocols [Medical Subject Headings]
Epidermal barrier function
Cicatrización de heridas
Anatomy::Cells::Connective Tissue Cells::Fibroblasts [Medical Subject Headings]
integumentary system
Chemicals and Drugs::Inorganic Chemicals::Sodium Compounds::Sodium Chloride [Medical Subject Headings]
General Medicine
Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Infective Agents
Local [Medical Subject Headings]
030220 oncology & carcinogenesis
Regenerative medicine
Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agents [Medical Subject Headings]
food.ingredient
Antiseptic/antibiotic testing
medicine.drug_class
Wound healing
regenerative medicine
Chemicals and Drugs::Carbohydrates::Polysaccharides::Glycosaminoglycans::Hyaluronic Acid [Medical Subject Headings]
Artificial skin
Anatomy::Cells::Epithelial Cells::Keratinocytes [Medical Subject Headings]
Article
03 medical and health sciences
food
Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival [Medical Subject Headings]
bioengineered artificial skin substitute
Viability assay
cell viability
Anatomy::Tissues::Epithelium [Medical Subject Headings]
Transepidermal water loss
epidermal barrier function
business.industry
lcsh:R
antiseptic/antibiotic testing
chemistry
Chemicals and Drugs::Polycyclic Compounds::Macrocyclic Compounds::Peptides
Cyclic::Polymyxins::Colistin [Medical Subject Headings]
Bioengineered artificial skin substitute
Células epidérmicas
sense organs
business
Zdroj: Journal of Clinical Medicine
Digibug: Repositorio Institucional de la Universidad de Granada
Universidad de Granada (UGR)
Volume 10
Issue 4
Journal of Clinical Medicine, Vol 10, Iss 642, p 642 (2021)
Digibug. Repositorio Institucional de la Universidad de Granada
instname
ISSN: 2077-0383
Popis: Bioengineered artificial skin substitutes (BASS) are the main treatment used in addition to autografts when skin injuries involve a large body surface area. Antiseptic/antibiotic treatment is necessary to prevent infections in the BASS implant area. This study aims to evaluate the effect of antiseptics and antibiotics on cell viability, structural integrity, and epidermal barrier function in BASS based on hyaluronic acid during a 28 day follow-up period. Keratinocytes (KTs) and dermal fibroblasts (DFs) were isolated from skin samples and used to establish BASS. The following antibiotic/antiseptic treatment was applied every 48 h: colistin (1%), chlorhexidine digluconate (1%), sodium chloride (0.02%), and polyhexanide (0.1%). Cell viability (LIVE/DEAD® assay), structural integrity (histological evaluation), and epidermal barrier function (trans-epidermal water loss, (TEWL), Tewameter®) were also evaluated. Cell viability percentage of BASS treated with chlorhexidine digluconate was significantly lower (p 0.001) than the other antiseptics at day 28. Compared to other treatments, chlorhexidine digluconate and polyhexanide significantly affected the epithelium. No significant differences were found regarding epidermal barrier. These results may be useful for treatment protocols after implantation of BASS in patients and evaluating them in clinical practice. BASS represent a suitable model to test in vitro the impact of different treatments of other skin wounds.
Ministry of Science, Innovation and Universities of Spain
University of Granada
Instituto de Salud Carlos III PI13/02576 PI17/02083
European Regional Development Fund "A way to make Europe"
Andalusian Regional Government SAS PI-0458-2016
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje