Anterior Gradient Protein 3 and S100 Calcium-Binding Protein P Levels in Different Endometrial Epithelial Compartments May Play an Important Role in Recurrent Pregnancy Failure

Autor: Andrew J. Drakeley, Dharani K. Hapangama, Nicola Tempest, Christopher J. Hill, J Drury, Hannan Al-Lamee, Elizabeth Batchelor
Rok vydání: 2021
Předmět:
0301 basic medicine
Endometrium
lcsh:Chemistry
0302 clinical medicine
Pregnancy
lcsh:QH301-705.5
Spectroscopy
media_common
AGR3
fertility
030219 obstetrics & reproductive medicine
Embryo
General Medicine
Middle Aged
Immunohistochemistry
Neoplasm Proteins
Computer Science Applications
medicine.anatomical_structure
window of implantation
Female
Adult
Abortion
Habitual
media_common.quotation_subject
Models
Biological
Article
Catalysis
reproduction
Inorganic Chemistry
Andrology
03 medical and health sciences
Stroma
Decidua
medicine
Humans
Physical and Theoretical Chemistry
Molecular Biology
Menstrual Cycle
Menstrual cycle
business.industry
Calcium-Binding Proteins
Organic Chemistry
Embryogenesis
medicine.disease
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
Carrier Proteins
business
Immunostaining
S100P
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 8
International Journal of Molecular Sciences, Vol 22, Iss 3835, p 3835 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22083835
Popis: Recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) are distressing conditions without effective treatments. The luminal epithelium (LE) is integral in determining receptivity of the endometrium, whereas functionalis glands and stroma aid in nurturing early embryo development. Calcium signalling pathways are known to be of vital importance to embryo implantation and pregnancy establishment, and anterior gradient protein 3 (AGR3) and S100 calcium-binding protein P (S100P) are involved with these pathways. We initially examined 20 full-thickness endometrial biopsies from premenopausal women across the menstrual cycle to characterize levels of AGR3 protein in each endometrial sub-region at the cellular level. A further 53 endometrial pipelle biopsies collected in the window of implantation were subsequently assessed to determine differential endometrial AGR3 and S100P levels relevant to RIF (n = 13) and RPL (n = 10) in comparison with parous women (n = 30) using immunohistochemistry. Significantly higher AGR3 and S100P immunostaining was observed in ciliated cells of the LE of women with recurrent reproductive failure compared with parous women, suggesting aberrant subcellular location-associated pathophysiology for these conditions. The nuclear localisation of S100P may allow transcriptional regulatory function, which is necessary for implantation of a viable pregnancy. Further work is thus warranted to assess their utility as diagnostic/therapeutic targets.
Databáze: OpenAIRE
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