NDM-1-producing Klebsiella pneumoniae isolated in the Sultanate of Oman
| Autor: | Sandrine Bernabeu, Fatma Al Rashdi, Patrice Nordmann, Laurent Poirel, Zaina Al Maskari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Oman Klebsiella pneumoniae Sequence analysis Microbial Sensitivity Tests Polymerase Chain Reaction beta-Lactamases Microbiology 03 medical and health sciences Plasmid Bacterial Proteins Drug Resistance Multiple Bacterial RNA Ribosomal 16S Pulsed-field gel electrophoresis Humans Pharmacology (medical) 030304 developmental biology Pharmacology 0303 health sciences biology 030306 microbiology Sequence Analysis DNA biochemical phenomena metabolism and nutrition Ribosomal RNA 16S ribosomal RNA biology.organism_classification Anti-Bacterial Agents Klebsiella Infections 3. Good health New Delhi metallo-beta-lactamase 1 Infectious Diseases biology.protein Multilocus sequence typing Plasmids |
| Zdroj: | Journal of Antimicrobial Chemotherapy; Vol 66 Journal of Antimicrobial Chemotherapy |
| ISSN: | 0305-7453 |
| DOI: | 10.1093/jac/dkq428 |
| Popis: | Objectives To analyse the mechanisms responsible for multidrug resistance in two carbapenem-resistant Klebsiella pneumoniae isolates recovered from patients hospitalized in Oman. Methods PCR and sequencing were used to search for β-lactamase and 16S RNA methylase genes. Multilocus sequence typing was used to determine the sequence type (ST) of each isolate. Clonal relationships were evaluated by PFGE. Results Both isolates carried the bla(NDM-1) carbapenemase gene. Isolate 601 was recovered from a patient who was transferred from India, whereas isolate 419 was from an Omani patient who had not travelled abroad. The two isolates were clonally unrelated, and belonged to ST14 (isolate 601) and ST340 (isolate 419). In addition to NDM-1, the ST14 isolate expressed β-lactamases CTX-M-15, SHV-28, OXA-1, OXA-9 and TEM-1, and the aminoglycoside resistance methylase ArmA. The ST340 isolate expressed β-lactamases SHV-11, OXA-1 and ArmA. In both isolates, the bla(NDM-1) gene was located on plasmids that were of similar size (170 kb), but of different incompatibility groups. Conclusion This is the first description of NDM-1 producers in the Arabian peninsula and in the Middle East. |
| Databáze: | OpenAIRE |
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